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Integrin beta-4 Polyclonal Antibody

View all (37) CD104 (Integrin beta 4) antibodies

Questions & Answers

Questions & Answers

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Integrin beta-4 Antibody (21738-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue slide using (Product # 21738-1-AP) Integrin beta-4 antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Integrin beta-4 Antibody in Western Blot (WB)

Integrin beta-4 Antibody in Western Blot (WB)

Integrin beta-4 Antibody in Western Blot (WB)

Integrin beta-4 Antibody in Western Blot (WB)

Integrin beta-4 Antibody in Flow Cytometry (Flow)

Integrin beta-4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue slide using (Product # 21738-1-AP) Integrin beta-4 antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue slide using (Product # 21738-1-AP) Integrin beta-4 antibody at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

Integrin beta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

Integrin beta-4 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 21738-1-AP) Integrin beta-4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Integrin beta-4 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Integrin beta-4 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Integrin beta-4 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Integrin beta-4 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Integrin beta-4 Antibody in Immunoprecipitation (IP) — IP result of anti-Integrin beta-4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

Product Details

21738-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Integrin beta-4 Fusion Protein Ag16450 (27-354 aa encoded by BC126411)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ANRCKKAPV KSCTECVRVD KDCAYCTDEM FRDRRCNTQA ELLAAGCQRE SIVVMESSFQ ITEETQIDTT LRRSQMSPQG LRVRLRPGEE RHFELEVFEP LESPVDLYIL MDFSNSMSDD LDNLKKMGQN LARVLSQLTS DYTIGFGKFV DKVSVPQTDM RPEKLKEPWP NSDPPFSFKN VISLTEDVDE FRNKLQGERI SGNLDAPEGG FDAILQTAVC TRDIGWRPDS THLLVFSTES AFHYEADGAN VLAGIMSRND ERCHLDTTGT YTQYRTQDYP SVPTLVRLLA KHNIIPIFAV TNYSYSYYEK LHTYFPVSSL GVLQEDSSN (27-354 aa encoded by BC126411)

Target Information

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Inhibition of the SLC35B2-TPST2 Axis of Tyrosine Sulfation Attenuates the Growth and Metastasis of Pancreatic Ductal Adenocarcinom.

Cellular and molecular gastroenterology and hepatology

Cai X,Li S,Zeng X,Xu M,Wang Z,Singhi AD,Tang D,Li S,Yates NA,Yang D,Xie W

Tue Aug 22 00:00:00 EDT 2023

Identification and Validation of Anoikis-Related Signatures for Predicting Prognosis in Lung Adenocarcinoma with Machine Learning.

International journal of general medicine

Wang Q,Sun N,Zhang M

Tue May 23 00:00:00 EDT 2023

Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Chen C,Chen J,Wang Y,Fang L,Guo C,Sang T,Peng H,Zhao Q,Chen S,Lin X,Wang X

Wed Feb 01 00:00:00 EST 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 EST 2023

Lack of NPR1 Increases Vascular Endothelial Adhesion through Induction of Integrin Beta 4.

International journal of molecular sciences

Liu H,Liu J,Long C,Chen L,Zhan W,Xiao W,Gong X,Liu M,Tian XL,Chen S

Thu Oct 20 00:00:00 EDT 2022

Lack of NPR1 Increases Vascular Endothelial Adhesion through Induction of Integrin Beta 4.

International journal of molecular sciences

Liu H,Liu J,Long C,Chen L,Zhan W,Xiao W,Gong X,Liu M,Tian XL,Chen S

Thu Oct 20 00:00:00 EDT 2022

Lack of NPR1 Increases Vascular Endothelial Adhesion through Induction of Integrin Beta 4.

International journal of molecular sciences

Liu H,Liu J,Long C,Chen L,Zhan W,Xiao W,Gong X,Liu M,Tian XL,Chen S

Thu Oct 20 00:00:00 EDT 2022

Lack of NPR1 Increases Vascular Endothelial Adhesion through Induction of Integrin Beta 4.

International journal of molecular sciences

Liu H,Liu J,Long C,Chen L,Zhan W,Xiao W,Gong X,Liu M,Tian XL,Chen S

Thu Oct 20 00:00:00 EDT 2022

Activation of GRP78 ATPase suppresses A549 lung cancer cell migration by promoting ITGB4 degradation.

Cell adhesion & migration

Ning J,Cui X,Li N,Li N,Zhao B,Miao J,Lin Z

Thu Dec 01 00:00:00 EST 2022

METTL14-mediated N⁶-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.

Cell communication and signaling : CCS

Liu Z,Sun T,Piao C,Zhang Z,Kong C

Sat Mar 19 00:00:00 EDT 2022

METTL14-mediated N⁶-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.

Cell communication and signaling : CCS

Liu Z,Sun T,Piao C,Zhang Z,Kong C

Sat Mar 19 00:00:00 EDT 2022

Single-Cell RNA-Seq Reveals Heterogeneity of Cell Communications between Schwann Cells and Fibroblasts within Vestibular Schwannoma Microenvironment.

The American journal of pathology

Xu M,Wang S,Jiang Y,Wang J,Xiong Y,Dong W,Yao Q,Xing Y,Liu F,Chen Z,Yu D

Thu Sep 01 00:00:00 EDT 2022

Gelatin-grafted tubular asymmetric scaffolds promote ureteral regeneration via activation of the integrin/Erk signaling pathway.

Frontiers in bioengineering and biotechnology

Song B,Fang L,Mao X,Ye X,Yan Z,Ma Q,Shi Z,Hu Y,Zhu Y,Cheng Y

Wed Feb 01 00:00:00 EST 2023

p63 Directs Subtype-Specific Gene Expression in HPV+ Head and Neck Squamous Cell Carcinoma.

Frontiers in oncology

Glathar AR,Oyelakin A,Gluck C,Bard J,Sinha S

Sat Jul 16 00:00:00 EDT 2022

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

International journal of oncology

Xu J,Lu W

Sat Aug 01 00:00:00 EDT 2020

17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death.

Journal of cellular biochemistry

Li Y,Chen Y,Qiu C,Ma X,Lei K,Cai G,Liang X,Liu J

Fri Feb 01 00:00:00 EST 2019

17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death.

Journal of cellular biochemistry

Li Y,Chen Y,Qiu C,Ma X,Lei K,Cai G,Liang X,Liu J

Fri Feb 01 00:00:00 EST 2019

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Journal of experimental & clinical cancer research : CR

Ma B,Zhang L,Zou Y,He R,Wu Q,Han C,Zhang B

Fri Jan 18 00:00:00 EST 2019

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Journal of experimental & clinical cancer research : CR

Ma B,Zhang L,Zou Y,He R,Wu Q,Han C,Zhang B

Fri Jan 18 00:00:00 EST 2019

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Journal of experimental & clinical cancer research : CR

Ma B,Zhang L,Zou Y,He R,Wu Q,Han C,Zhang B

Fri Jan 18 00:00:00 EST 2019

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Journal of experimental & clinical cancer research : CR

Ma B,Zhang L,Zou Y,He R,Wu Q,Han C,Zhang B

Fri Jan 18 00:00:00 EST 2019

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.

Oncogene

Wang S,Li J,Xie J,Liu F,Duan Y,Wu Y,Huang S,He X,Wang Z,Wu X

Sun Jul 01 00:00:00 EDT 2018

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Scientific reports

Li XL,Liu L,Li DD,He YP,Guo LH,Sun LP,Liu LN,Xu HX,Zhang XP

Fri Jan 13 00:00:00 EST 2017

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Scientific reports

Li XL,Liu L,Li DD,He YP,Guo LH,Sun LP,Liu LN,Xu HX,Zhang XP

Fri Jan 13 00:00:00 EST 2017

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Scientific reports

Li XL,Liu L,Li DD,He YP,Guo LH,Sun LP,Liu LN,Xu HX,Zhang XP

Fri Jan 13 00:00:00 EST 2017

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Scientific reports

Li XL,Liu L,Li DD,He YP,Guo LH,Sun LP,Liu LN,Xu HX,Zhang XP

Fri Jan 13 00:00:00 EST 2017

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Scientific reports

Li XL,Liu L,Li DD,He YP,Guo LH,Sun LP,Liu LN,Xu HX,Zhang XP

Fri Jan 13 00:00:00 EST 2017

Bioinformatics

Protein Aliases: CD antigen CD104; CD104; CD104 antigen; GP150; Integrin beta-4

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Gene Aliases: AA407042; C230078O20; CD104; GP150; ITGB4

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UniProt ID: (Human) P16144, (Mouse) A2A863, (Rat) Q64632

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Entrez Gene ID: (Human) 3691, (Mouse) 192897, (Rat) 25724

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G-protein coupled receptor binding receptor activity protein binding insulin-like growth factor I binding neuregulin binding cell adhesion molecule binding integrin

Process(es)

autophagy cell adhesion cell-matrix adhesion integrin-mediated signaling pathway response to wounding extracellular matrix organization hemidesmosome assembly nail development skin development mesodermal cell differentiation digestive tract development cell motility renal system development amelogenesis cell communication filopodium assembly multicellular organism development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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