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Invitrogen
Involucrin Monoclonal Antibody (SY8)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Involucrin Antibody (MA1-90573) in WB
Western blot was performed using Anti-Involucrin Monoclonal Antibody (SY8)(Product # MA1-90573) and a 68kDa band corresponding to Involucrin was observed across skin derived cell lines (HaCaT and A-431) and not in others. Whole Cell Extract-WCL (30 µg lysate) of HaCaT (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MDA-MB-231 (Lane 4), Raji (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting S... View More
Product Details
MA1-90573
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Dog,
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SY8
Immunogen
Pure involucrin from human keratinocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2249265
Product Specific Information
MA1-90573 detects Involucrin from non-human primate, porcine, and human samples.
Target Information
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Involucrin
Gene Aliases: IVL
UniProt ID: (Human) P07476, (Pig) P18175
Entrez Gene ID: (Dog) 102152041, (Human) 3713, (Pig) 407242
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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