Proteintech

JAK1 Monoclonal Antibody (3H7A8)

JAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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JAK1 Antibody (66466-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

66466-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3H7A8

Immunogen

JAK1 Fusion Protein Ag17940 (8-363 aa encoded by BC132729)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EDCNAMAFC AKMRSSKKTE VNLEAPEPGV EVIFYLSDRE PLRLGSGEYT AEELCIRAAQ ACRISPLCHN LFALYDENTK LWYAPNRTIT VDDKMSLRLH YRMRFYFTNW HGTNDNEQSV WRHSPKKQKN GYEKKKIPDA TPLLDASSLE YLFAQGQYDL VKCLAPIRDP KTEQDGHDIE NECLGMAVLA ISHYAMMKKM QLPELPKDIS YKRYIPETLN KSIRQRNLLT RMRINNVFKD FLKEFNNKTI CDSSVSTHDL KVKYLATLET LTKHYGAEIF ETSMLLISSE NEMNWFHSND GGNVLYYEVM VTGNLGIQWR HKPNVVSVEK EKNKLKRKKL ENKHKKDEEK NKIREEW (8-363 aa encoded by BC132729)

Target Information

Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells.

In vitro cellular & developmental biology. Animal

Pan C,Fei Q,Jin J,Zheng J,Wu D,Li H,Huang X,Kong X

Sun Jan 01 00:00:00 EST 2023

Knockdown of Brg1 reduced mucus secretion in HDM stimulated airway inflammation.

Molecular immunology

Xu M,Hu J,Yang L,Gen G,Fu Z,Luo Z,Zou W

Sun Jan 01 00:00:00 EST 2023

EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Pan Y,Shu G,Fu L,Huang K,Zhou X,Gui C,Liu H,Jin X,Chen M,Li P,Cen J,Feng Z,Lu J,Chen Z,Li J,Xu Q,Wang Y,Liang H,Wang Z,Deng Q,Chen W,Luo J,Yang J,Zhang J,Wei J

Sat Apr 01 00:00:00 EDT 2023

LIF Inhibits Proliferation of Esophageal Squamous Carcinoma Cells by Radiation Mediated Through JAK-STAT Signaling Pathway.

Journal of Cancer

Luo H,Yang Z,Zhang Q,Li T,Liu R,Feng S,Liu Z,Sun S,Chen J,Wang X,Xie X

Sat Apr 15 00:00:00 EDT 2023

The Protective Role of Scorias spongiosa Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice.

Foods (Basel, Switzerland)

Xu Y,Feng H,Zhang Z,Zhang Q,Tang J,Zhou J,Wang Y,Peng W

Fri Feb 03 00:00:00 EST 2023

P53 Deficiency Accelerate Esophageal Epithelium Intestinal Metaplasia Malignancy.

Biomedicines

Qiu Q,Guo G,Guo X,Hu X,Yu T,Liu G,Zhang H,Chen Y,She J

Mon Mar 13 00:00:00 EDT 2023

IFN-γ enhances CLL cell resistance to ABT-199 by regulating MCL-1 and BCL-2 expression via the JAK-STAT3 signaling pathway.

Leukemia & lymphoma

Shao X,Meng X,Yang H,Wang X,Qin L,Shen G,Xi X,Zhao H,Macip S,Chen Y

Sun Jan 01 00:00:00 EST 2023

IFNγ induces Bcl3 expression by JAK1/STAT1/p65 signaling, resulting in increased IL-8 expression in ovarian cancer cells.

FEBS open bio

Gaire B,Padmanabhan S,Zou Y,Uddin MM,Reddy SU,Vancurova I

Tue Aug 01 00:00:00 EDT 2023

IFNγ-induced PD-L1 expression in ovarian cancer cells is regulated by JAK1, STAT1 and IRF1 signaling.

Cellular signalling

Padmanabhan S,Gaire B,Zou Y,Uddin MM,Vancurova I

Thu Sep 01 00:00:00 EDT 2022

IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4.

Investigational new drugs

Huo J,Fu L,Jin M,Li Z,Zhang M

Wed Jun 01 00:00:00 EDT 2022

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice.

Antioxidants (Basel, Switzerland)

Lu S,Xu S,Chen L,Deng Y,Feng J

Tue Sep 13 00:00:00 EDT 2022

Cannabis suppresses antitumor immunity by inhibiting JAK/STAT signaling in T cells through CNR2.

Signal transduction and targeted therapy

Xiong X,Chen S,Shen J,You H,Yang H,Yan C,Fang Z,Zhang J,Cai X,Dong X,Kang T,Li W,Zhou P

Wed Apr 06 00:00:00 EDT 2022

The Anti-Inflammatory and Anti-Pruritus Mechanisms of Huanglian Jiedu Decoction in the Treatment of Atopic Dermatitis.

Frontiers in pharmacology

Xu Y,Chen S,Zhang L,Chen G,Chen J

Tue Dec 21 00:00:00 EST 2021

Triptolide reduces salivary gland damage in a non-obese diabetic mice model of Sjögren's syndrome via JAK/STAT and NF-κB signaling pathways.

Journal of clinical biochemistry and nutrition

Guo Y,Ji W,Lu Y,Wang Y

Mon Mar 01 00:00:00 EST 2021

Study on the Molecular Basis of Huanglian Jiedu Decoction Against Atopic Dermatitis Integrating Chemistry, Biochemistry, and Metabolomics Strategies.

Frontiers in pharmacology

Chen J,Chen S,Chen J,Shen B,Jiang Z,Xu Y

Sat Jan 01 00:00:00 EST 2022

Polygalaxanthone III downregulates inflammation in the lipopolysaccharide-stimulated RAW264.7 macrophages: A quantibody array analysis.

Journal of pharmacological sciences

Wu Y,Fu H,Yang X,Leng F,Huang Y,Deng H,Xiang Q,Zhang S

Fri Oct 01 00:00:00 EDT 2021

CXCL10 Produced by HPV-Positive Cervical Cancer Cells Stimulates Exosomal PDL1 Expression by Fibroblasts via CXCR3 and JAK-STAT Pathways.

Frontiers in oncology

Chen X,He H,Xiao Y,Hasim A,Yuan J,Ye M,Li X,Hao Y,Guo X

Tue Aug 24 00:00:00 EDT 2021

A network pharmacology approach to reveal the pharmacological targets and biological mechanism of compound kushen injection for treating pancreatic cancer based on WGCNA and in vitro experiment validation.

Chinese medicine

Wu C,Huang ZH,Meng ZQ,Fan XT,Lu S,Tan YY,You LM,Huang JQ,Stalin A,Ye PZ,Wu ZS,Zhang JY,Liu XK,Zhou W,Zhang XM,Wu JR

Mon Nov 22 00:00:00 EST 2021

Increased expression of IFN-γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop.

Experimental and therapeutic medicine

Liu H,Wang W,Liu C

Mon Feb 01 00:00:00 EST 2021

CCL21/CCR7 interaction promotes EMT and enhances the stemness of OSCC via a JAK2/STAT3 signaling pathway.

Journal of cellular physiology

Chen Y,Shao Z,Jiang E,Zhou X,Wang L,Wang H,Luo X,Chen Q,Liu K,Shang Z

Tue Sep 01 00:00:00 EDT 2020

Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response.

Oxidative medicine and cellular longevity

Wang G,Xu B,Shi F,Du M,Li Y,Yu T,Chen L

Mon Dec 16 00:00:00 EST 2019

Integration of transcriptomics and network analysis deciphers the mechanisms of baicalein in improving learning and memory impairment in senescence-accelerated mouse prone 8 (SAMP8).

European journal of pharmacology

Li J,Zhou Y,Du G,Qin X,Gao L

Sun Dec 15 00:00:00 EST 2019

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 EDT 2019

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 EDT 2019

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Cells

Li M,Gao J,Li D,Yin Y

Wed Aug 08 00:00:00 EDT 2018

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Cells

Li M,Gao J,Li D,Yin Y

Wed Aug 08 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: EC 2.7.10.2; JAK-1; Janus kinase 1; Janus protein tyrosine kinase 1; kinase Jak1; Tyrosine-protein kinase JAK1

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Gene Aliases: AA960307; BAP004; C130039L05Rik; JAK1; JAK1A; JAK1B; JTK3

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UniProt ID: (Human) P23458

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Entrez Gene ID: (Human) 3716, (Rat) 84598, (Mouse) 16451

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Function(s)

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity receptor binding growth hormone receptor binding protein binding ATP binding protein phosphatase binding ubiquitin protein ligase binding CCR5 chemokine receptor binding metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway cell differentiation intracellular signal transduction peptidyl-tyrosine autophosphorylation interleukin-2-mediated signaling pathway regulation of cell proliferation innate immune response response to antibiotic protein autophosphorylation MAPK cascade protein phosphorylation positive regulation of GTPase activity interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway type I interferon signaling pathway regulation of type I interferon-mediated signaling pathway positive regulation of sprouting angiogenesis phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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JAK1 Monoclonal Antibody (3H7A8)

JAK1 Antibody (66466-1-IG) in IHC (P)

Product Details

66466-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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