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Invitrogen
JMJD2A Recombinant Rabbit Monoclonal Antibody (6H56L3)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
JMJD2A Antibody (702449) in ICC/IF
For immunofluorescence analysis, Ntera2 cells were fixed and permeabilized for detection of endogenous KDM4A using Anti- KDM4A Recombinant Rabbit Monoclonal Antibody (Product # 702449, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KDM4A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3... View More
Product Details
702449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H56L3
Immunogen
Protein corresponding to human (aa379-aa628)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2688041
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(36) demethylase 4A; [histone H3]-trimethyl-L-lysine(9) demethylase 4A; JmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; Jumonji domain-containing protein 2A; lysine (K)-specific demethylase 4A; Lysine-specific demethylase 4A; tudor domain containing 14A
Gene Aliases: JHDM3A; JMJD2; JMJD2A; KDM4A; KIAA0677; TDRD14A
UniProt ID: (Human) O75164
Entrez Gene ID: (Human) 9682
transcription, DNA-templated
negative regulation of autophagy
negative regulation of gene expression
cardiac muscle hypertrophy in response to stress
viral process
histone demethylation
negative regulation of transcription, DNA-templated
oxidation-reduction process
histone H3-K36 demethylation
negative regulation of histone H3-K9 trimethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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