Invitrogen

JNK1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

14 References

View all (53) JNK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

JNK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

JNK1 Antibody (44-690G) in IHC (P)

Immunohistochemistry analysis of JNK1 showing staining in the cytoplasm of paraffin-embedded human prostate carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature... View More

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JNK1 Antibody — FIG. 7. Treatment with p38 inhibitors prevents ICP27 induced apoptosis. (A) Immunoblot analysis of SAPK phosphorylation in HTO-H cells. Cells were either left untreated or treated with sorbitol for 30 min. Cells were then treated with vehicle control (V/C; DMSO), p38 inhibitor (SB203580 at 5 muM), or JNK inhibitor (SP600125 at 1 muM) for 30 min as indicated. Following treatment, protein extracts were collected and analyzed by immunoblotting for p38, phosphorylated p38 (P-p38), JNK1, and phosphorylated JNK1 and JNK2 (P-JNK1 and P-JNK2, respectively). (B to G) Immunofluorescence analysis of caspase-3 activation in cells expressing ICP27 treated with inhibitors. Uninduced TE51 cells were treated with vehicle control (V/C; DMSO) (B), p38 inhibitor (C), and JNK inhibitor (D). TE51 cells induced with doxycycline (2 mug/ml) were treated with DMSO (E), p38 inhibitor (F), and JNK inhibitor (G). Cells were fixed and processed 3 h posttreatment using antibodies specific for activated caspase-3 (red-Cy3) and ICP27 (green-Cy2). (H) Enumeration of the percentage of ICP27-positive cells staining positive for activated caspase-3 following treatment with vehicle control, p38 inhibitor, and JNK inhibitor (>1,200 cells counted for each condition). *, P < 0.05. Dox+, with doxycycline; Dox-, without doxycycline. {TM}

Product Details

44-690G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized peptide derived from an internal region of JNK1. This region is conserved among human, mouse, and rat forms of JNK1, JNK2, and JNK3 proteins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533724

Target Information

MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is also a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Evidence-based complementary and alternative medicine : eCAM

Cao YJ,Li HZ,Zhao J,Sun YM,Jin XW,Lv SQ,Luo JY,Fang XX,Wen WB,Liao JB

44-690G was used in Western Blotting to investigate the mechanisms by which Jian-Gan-Xiao-Zhi decoction improve nonalcoholic fatty liver disease.

Tue Jul 19 00:00:00 EDT 2022

Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing.

Nature

Mahmoudi S,Mancini E,Xu L,Moore A,Jahanbani F,Hebestreit K,Srinivasan R,Li X,Devarajan K,Prélot L,Ang CE,Shibuya Y,Benayoun BA,Chang ALS,Wernig M,Wysocka J,Longaker MT,Snyder MP,Brunet A

44-690G was used in Western Blotting to show that this variability may reflect distinct stochastic ageing trajectories between individuals, and could help in developing personalized strategies to improve iPS cell generation and wound healing in elderly individuals.

Tue Oct 01 00:00:00 EDT 2019

Expression of cFLIPL Determines the Basal Interaction of Bcl-2 With Beclin-1 and Regulates p53 Dependent Ubiquitination of Beclin-1 During Autophagic Stress.

Journal of cellular biochemistry

Ranjan K,Pathak C

44-690G was used in western blot to study regulation of p53 dependent ubiquitination of Beclin-1 during autophagic stress and basal interaction of Bcl-2 with Beclin-1 by expression of cFLIPL

Mon Aug 01 00:00:00 EDT 2016

Expression of FADD and cFLIP_L balances mitochondrial integrity and redox signaling to substantiate apoptotic cell death.

Molecular and cellular biochemistry

Ranjan K,Pathak C

44690G was used in western blot to elucidate how FADD contributes to mitochondrial-associated apoptosis

Tue Nov 01 00:00:00 EDT 2016

FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis.

Scientific reports

Ranjan K,Pathak C

44-690G was used in western blot to show how FADD regulates NF-kappaB activation and promotes ubiquitination of cFLIPL, which induces apoptosis

Mon Mar 14 00:00:00 EDT 2016

Functional comparison of herpes simplex virus 1 (HSV-1) and HSV-2 ICP27 homologs reveals a role for ICP27 in virion release.

Journal of virology

Park D,Lalli J,Sedlackova-Slavikova L,Rice SA

44-690G was used in western blot to compare ICP27t2 and ICP27 from herpes simplex virus

Sun Mar 01 00:00:00 EST 2015

Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.

Biochimica et biophysica acta

Bernusso VA,Machado-Neto JA,Pericole FV,Vieira KP,Duarte AS,Traina F,Hansen MD,Olalla Saad ST,Barcellos KS

44-690G was used in western blot to study the effect of Imatinib on VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.

Sun Feb 01 00:00:00 EST 2015

Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ

44-690G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.

Sun Jun 01 00:00:00 EDT 2014

Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.

BMC immunology

Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W

Wed Mar 14 00:00:00 EDT 2012

Herpes simplex virus type 1 ICP27 induces p38 mitogen-activated protein kinase signaling and apoptosis in HeLa cells.

Journal of virology

Gillis PA,Okagaki LH,Rice SA

Published figure using JNK1 polyclonal antibody (Product # 44-690G) in Western Blot

Sun Feb 01 00:00:00 EST 2009

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway.

Journal of virology

Zapata HJ,Nakatsugawa M,Moffat JF

44-690G was used in western blot to study the effect of infection with varicella-zoster virus on c-Jun N-terminal kinase signaling

Mon Jan 01 00:00:00 EST 2007

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Gynecologic oncology

Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R

Fri Sep 01 00:00:00 EDT 2006

Homozygous deletion of MKK4 in ovarian serous carcinoma.

Cancer biology & therapy

Nakayama K,Nakayama N,Davidson B,Katabuchi H,Kurman RJ,Velculescu VE,Shih IeM,Wang TL

44-690G was used in immunohistochemistry - paraffin section to search for chromosomal deletions in ovarian serous carcinoma and identify genes that could contribute to disease

Thu Jun 01 00:00:00 EDT 2006

Matrix metalloproteinases (MMP), EMMPRIN (extracellular matrix metalloproteinase inducer) and mitogen-activated protein kinases (MAPK): co-expression in metastatic serous ovarian carcinoma.

Clinical & experimental metastasis

Davidson B,Givant-Horwitz V,Lazarovici P,Risberg B,Nesland JM,Trope CG,Schaefer E,Reich R

Thu Dec 18 00:00:00 EST 2003

Bioinformatics

Protein Aliases: c-Jun N-terminal kinase 1; FLJ12099; FLJ33785; JNK-46; JNK1 beta1 protein kinase; JNK3; JNK3A; JUN N-terminal kinase; MAP kinase 8; MAPK 10; MAPK 8; MAPK10; MGC50974; mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; MK10; OTTHUMP00000019552; p493F12; p54 gamma; p54bSAPK; Phospho-JNK; PRKM10; protein kinase JNK1; protein kinase mitogen-activated 8; SAPK gamma; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1

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Gene Aliases: AI849689; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; MAPK8; PRKM8; SAPK1; SAPK1C

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UniProt ID: (Human) P45983, (Rat) P49185, (Mouse) Q91Y86

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Entrez Gene ID: (Human) 5599, (Rat) 116554, (Mouse) 26419

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Function(s)

protein serine/threonine kinase activity JUN kinase activity protein binding ATP binding kinase activity enzyme binding histone deacetylase regulator activity histone deacetylase binding nucleotide binding protein kinase activity MAP kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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JNK1 Polyclonal Antibody

JNK1 Antibody (44-690G) in IHC (P)

Product Details

44-690G

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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