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JNK3 (MAPK10) Antibody (CF813292) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (RC207216, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1 (1:500).
Product Details
CF813292
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI10D8
Immunogen
Human recombinant protein fragment corresponding to amino acids 39-402 of human JNK1 (NP_002744) produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
MAPK10 (JNK3) is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). MAPK10 is involved in neuronal apoptosis and phosphorylates MAP2K4. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (NhpK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Jun N-terminal kinase 3; JNK3 alpha protein kinase; JNK3 beta1 protein kinase; JNK3 beta2 protein kinase; MAP kinase 10; MAP kinase p49 3F12; MAPK 10; mitogen activated protein kinase 10; Mitogen-activated protein kinase 10; OTTHUMP00000161181; OTTHUMP00000161183; OTTHUMP00000218718; p54-beta; SAPK-beta; SAPK/Erk/kinase 2; SAPK1b; Stress activated protein kinase beta; Stress-activated protein kinase 1b; Stress-activated protein kinase JNK3
Gene Aliases: C230008H04Rik; JNK3; JNK3A; JNK3B1; JNK3B2; MAPK10; p493F12; p54bSAPK; PRKM10; SAPb; SAPK(beta); SAPK1B; SAPKC; Serk2
UniProt ID: (Human) P53779, (Mouse) Q61831, (Rat) P49187
Entrez Gene ID: (Human) 5602, (Mouse) 26414, (Rat) 25272
JUN kinase activity
MAP kinase kinase activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
activation of MAPK activity
protein phosphorylation
signal transduction
JNK cascade
JUN phosphorylation
response to light stimulus
Fc-epsilon receptor signaling pathway
regulation of circadian rhythm
rhythmic process
regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription from RNA polymerase II promoter
phosphorylation
locomotor rhythm
MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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