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Jagged1 Antibody (PA5-47804) in Neu
Neutralization of Jagged1 in C3H10T1/2 mouse embryonic fibroblast cell line. Samples were incubated in Jagged1 polyclonal antibody (Product # PA5-47804). Recombinant Rat Jagged 1 Fc Chimera induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP‚2 in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP‚2 (150 ng/mL) is neutralized (g... View More
Product Details
PA5-47804
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Jagged1 Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607338
Product Specific Information
In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD339; jagged canonical Notch ligand 1; Jagged1; Protein jagged-1
Gene Aliases: Jag1
UniProt ID: (Rat) Q63722
Entrez Gene ID: (Rat) 29146
cell fate determination
blood vessel remodeling
morphogenesis of an epithelial sheet
T cell mediated immunity
pulmonary valve morphogenesis
cardiac right ventricle morphogenesis
Notch signaling pathway
organ morphogenesis
organ regeneration
response to muramyl dipeptide
aorta morphogenesis
regulation of cell proliferation
auditory receptor cell differentiation
camera-type eye development
negative regulation of cell differentiation
negative regulation of fat cell differentiation
positive regulation of myeloid cell differentiation
negative regulation of neuron differentiation
positive regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
inner ear development
cardiac septum morphogenesis
ciliary body morphogenesis
pulmonary artery morphogenesis
cardiac neural crest cell development involved in outflow tract morphogenesis
Notch signaling involved in heart development
endocardial cushion cell development
distal tubule development
loop of Henle development
neuronal stem cell population maintenance
negative regulation of stem cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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