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K-Ras Antibody (PA5-70104) in ICC/IF
Immunofluorescence analysis of K-Ras was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with K-Ras Rabbit Polyclonal Antibody (Product # PA5-70104) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
Product Details
PA5-70104
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the middle region of human K-Ras
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2689573
Product Specific Information
This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; GTPase KRas; K RAS; K-Ras 2; Ki-Ras; Kirsten rat sarcoma viral oncogene homologue 2 (active); RASK; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Aliases: c-Ki-ras; Kras; Kras2; p21
UniProt ID: (Rat) P08644
Entrez Gene ID: (Rat) 24525
positive regulation of protein phosphorylation
small GTPase mediated signal transduction
Ras protein signal transduction
positive regulation of cell proliferation
visual learning
positive regulation of gene expression
cytokine-mediated signaling pathway
actin cytoskeleton organization
regulation of synaptic transmission, GABAergic
positive regulation of Rac protein signal transduction
social behavior
positive regulation of MAP kinase activity
negative regulation of neuron apoptotic process
negative regulation of cell differentiation
regulation of long-term neuronal synaptic plasticity
homeostasis of number of cells within a tissue
positive regulation of nitric-oxide synthase activity
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
response to glucocorticoid
response to mineralocorticoid
epithelial tube branching involved in lung morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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