Invitrogen

K-Ras Monoclonal Antibody (9.13)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

7 References

View all (50) K-Ras antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

K-Ras Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

K-Ras Antibody (415700) in ICC/IF

Immunofluorescence analysis of K-Ras was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with K-Ras (9.13) Mouse Monoclonal Antibody (Product # 415700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More

K-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

K-Ras Antibody — Figure 2 miR-200c inhibits K-ras protein expression without affecting KRAS mRNA levels A) Luciferase reporter assay with different breast cancer cell lines. The renilla luciferase reporter containing the 3'UTR of KRAS including the predicted target site of miR-200c (RLuc) or the firefy luciferase control plasmid pGL3 (FLuc) were transfected into the indicated cell lines. Renilla reporter luciferase activity was normalized to the activity of the firefy control as ratio (RLuc/FLuc). Values are stated as mean +- SEM (n=5). B) Luciferase reporter assay upon miR-200c modulation. MDA-MB-436 cells were transfected with either pre-miR-200c (pre) or scrambled pre-miR-control (ctr). BT-474 cells were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr). Relative luciferase activities (RLuc/FLuc) are depicted in the graph. Values are stated as mean +- SEM (n=5). For statistical analysis a student's t-test was performed (**p<0.01; ***p<0.001). C) miR-200c inhibition in the miR-200c high cell lines BT-474 and MCF-7. Indicated cell lines were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr) and at 72h post transfection subjected to Western blot analysis (upper panel) or quantitative RT-PCR (lower panel). Values are stated as mean +- SD (n=3). D) miR-200c overexpression in the miR-200c low cell lines MDA-MB-231 and MDA-MB-436. Indicated cell lines were transfected with either pre-miR-200c (pre) or scrambled pre-miR-contro {KD}

Product Details

415700

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Immunoprecipitation (IP)

5 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9.13

Immunogen

Immunogen corresponds to aa 170-180 from P01116-1 (isoform 2A) of human K-Ras.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532192

Product Specific Information

This antibody is specific for human K-Ras (K-Ras2, Ki-Ras, c-K-ras, GTPase KRas) protein (accession # NP_004976.2, P01116), which is 100% homologous with mouse, 95% with rat, and 94% with bovine, respectively. Reactivity has been confirmed on western blots with human HeLa and WI-38 cell lysates as well as rat KNRK and mouse NIH 3T3 cell lysates, and identifies the target band at~21 kDa. The reactivity has been also confirmed with rat KNRK cells using immunoprecipitation and with HeLa cells by immunofluorescence. Based on amino acid sequence homology, reactivity with bovine is also expected.

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KRAS-specific antibody binds to KRAS protein inside colorectal adenocarcinoma cells and inhibits its localization to the plasma membrane.

Frontiers in oncology

Lam KK,Low YS,Lo M,Wong M,Leong Tang C,Tan E,Chok AY,Seow-En I,Wong SH,Cheah PY

415700 was used in Sample prep to demonstrate that internalized anti-KRAS antibody inhibits KRAS function in tumor.

Sat Apr 15 00:00:00 EDT 2023

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Han K,Pierce SE,Li A,Spees K,Anderson GR,Seoane JA,Lo YH,Dubreuil M,Olivas M,Kamber RA,Wainberg M,Kostyrko K,Kelly MR,Yousefi M,Simpkins SW,Yao D,Lee K,Kuo CJ,Jackson PK,Sweet-Cordero A,Kundaje A,Gentles AJ,Curtis C,Winslow MM,Bassik MC

415700 was used in Western Blotting to reveal key differences between 2D and 3D cancer models, and establish a generalizable strategy for performing CRISPR screens in spheroids to reveal cancer vulnerabilities.

Wed Apr 01 00:00:00 EDT 2020

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Wed Apr 01 00:00:00 EDT 2020

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Published figure using K-Ras monoclonal antibody (Product # 415700) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Integrated analysis of patient samples identifies biomarkers for venetoclax efficacy and combination strategies in acute myeloid leukemia.

Nature cancer

Zhang H,Nakauchi Y,Köhnke T,Stafford M,Bottomly D,Thomas R,Wilmot B,McWeeney SK,Majeti R,Tyner JW

415700 was used in Western Blot to show that Venetoclax in combination with an MCL1 inhibitor AZD5991 induced synthetic lethality and circumvented venetoclax resistance.

Sat Aug 01 00:00:00 EDT 2020

A Novel KRAS Antibody Highlights a Regulation Mechanism of Post-Translational Modifications of KRAS during Tumorigenesis.

International journal of molecular sciences

Assi M,Pirlot B,Stroobant V,Thissen JP,Jacquemin P

415700 was used in Western Blotting to find that KRAS prenylation was increased ex vivo and in vivo in a model of KRASG12D-driven tumorigenesis, which was concomitant with an induction of expression of essential KRAS prenylation enzymes.

Wed Sep 02 00:00:00 EDT 2020

Targeting wild-type KRAS-amplified gastroesophageal cancer through combined MEK and SHP2 inhibition.

Nature medicine

Wong GS,Zhou J,Liu JB,Wu Z,Xu X,Li T,Xu D,Schumacher SE,Puschhof J,McFarland J,Zou C,Dulak A,Henderson L,Xu P,O'Day E,Rendak R,Liao WL,Cecchi F,Hembrough T,Schwartz S,Szeto C,Rustgi AK,Wong KK,Diehl JA,Jensen K,Graziano F,Ruzzo A,Fereshetian S,Mertins P,Carr SA,Beroukhim R,Nakamura K,Oki E,Watanabe M,Baba H,Imamura Y,Catenacci D,Bass AJ

415700 was used in Immunohistochemistry to examine focal high-level amplification of the KRAS gene in the absence of coding mutations, as a secondary means of KRAS activation in cancer.

Sun Jul 01 00:00:00 EDT 2018

The proto-oncogene KRAS is targeted by miR-200c.

Oncotarget

Kopp F,Wagner E,Roidl A

415700 was used in western blot to demonstrate that miR-200c regulates KRAS.

Wed Jan 15 00:00:00 EST 2014

Bioinformatics

Protein Aliases: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

View more View less

Gene Aliases: AI929937; C-K-RAS; c-Ki-ras; CFC2; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; p21; p21B; RALD; ras; RASK2

View more View less

UniProt ID: (Human) P01116, (Rat) P08644, (Mouse) P32883

View more View less

Entrez Gene ID: (Human) 3845, (Rat) 24525, (Mouse) 16653

View more View less

Function(s)

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding small GTPase

Process(es)

MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue leukocyte migration positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis small GTPase mediated signal transduction signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

K-Ras Monoclonal Antibody (9.13)

K-Ras Antibody (415700) in ICC/IF

Product Details

415700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!