Proteintech

KAP1 Polyclonal Antibody

KAP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

KAP1 Antibody (15202-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

KAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KAP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15202-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KAP1 Fusion Protein Ag7350 (484-835 aa encoded by BC004978)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD TKFSAVLVEP PPMSLPGAGL SSQELSGGPG DGP (484-835 aa encoded by BC004978)

Target Information

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression.

Oncogene

Li L,Wang X,Hu K,Liu X,Qiu L,Bai C,Cui Y,Wang B,Wang Z,Wang H,Cheng R,Hua J,Hai L,Wang M,Liu M,Song Z,Xiao C,Li B

Thu Jun 01 00:00:00 EDT 2023

ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression.

Oncogene

Li L,Wang X,Hu K,Liu X,Qiu L,Bai C,Cui Y,Wang B,Wang Z,Wang H,Cheng R,Hua J,Hai L,Wang M,Liu M,Song Z,Xiao C,Li B

Thu Jun 01 00:00:00 EDT 2023

Trim28 citrullination maintains mouse embryonic stem cell pluripotency via regulating Nanog and Klf4 transcription.

Science China. Life sciences

Zhang Y,Wan X,Qiu L,Zhou L,Huang Q,Wei M,Liu X,Liu S,Zhang B,Han J

Wed Mar 01 00:00:00 EST 2023

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

iScience

Ghanam AR,Ke S,Wang S,Elgendy R,Xie C,Wang S,Zhang R,Wei M,Liu W,Cao J,Zhang Y,Zhang Z,Xue T,Zheng Y,Song X

Fri Jan 20 00:00:00 EST 2023

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

iScience

Ghanam AR,Ke S,Wang S,Elgendy R,Xie C,Wang S,Zhang R,Wei M,Liu W,Cao J,Zhang Y,Zhang Z,Xue T,Zheng Y,Song X

Fri Jan 20 00:00:00 EST 2023

TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation.

Communications biology

Horvath RM,Dahabieh M,Malcolm T,Sadowski I

Mon Jan 23 00:00:00 EST 2023

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Experimental eye research

Zhang J,Gao H,Jiang N,Jing M,Sun Z,Du C,Zhang J,Wang M,Li J,Gao F,Hu Y,Mu H,Cui X

Fri Jul 01 00:00:00 EDT 2022

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Experimental eye research

Zhang J,Gao H,Jiang N,Jing M,Sun Z,Du C,Zhang J,Wang M,Li J,Gao F,Hu Y,Mu H,Cui X

Fri Jul 01 00:00:00 EDT 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 EST 2022

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Oncogene

Jin X,Zhang B,Zhang H,Yu H

Sat Jan 01 00:00:00 EST 2022

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Oncogene

Jin X,Zhang B,Zhang H,Yu H

Sat Jan 01 00:00:00 EST 2022

A method to map the interaction network of the nuclear lamina with genetically encoded photo-crosslinkers in vivo.

Frontiers in chemistry

Neumann-Staubitz P,Kitsberg D,Buxboim A,Neumann H

Sat Sep 17 00:00:00 EDT 2022

Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.

Nucleic acids research

Qin W,Ugur E,Mulholland CB,Bultmann S,Solovei I,Modic M,Smets M,Wierer M,Forné I,Imhof A,Cardoso MC,Leonhardt H

Wed Jul 21 00:00:00 EDT 2021

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

The Journal of experimental medicine

Ma L,Yu L,Jiang BC,Wang J,Guo X,Huang Y,Ren J,Sun N,Gao DS,Ding H,Lu J,Zhou H,Zou L,Gao Y,Wang L,Sun K,Ming Y,Meng Z,Tao YX,Yan M

Mon Dec 06 00:00:00 EST 2021

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

The Journal of experimental medicine

Ma L,Yu L,Jiang BC,Wang J,Guo X,Huang Y,Ren J,Sun N,Gao DS,Ding H,Lu J,Zhou H,Zou L,Gao Y,Wang L,Sun K,Ming Y,Meng Z,Tao YX,Yan M

Mon Dec 06 00:00:00 EST 2021

TRIM28 maintains genome imprints and regulates development of porcine SCNT embryos.

Reproduction (Cambridge, England)

Zhai Y,Zhang M,An X,Zhang S,Kong X,Li Q,Yu H,Dai X,Li Z

Thu Apr 01 00:00:00 EDT 2021

TRIM28 Expression on Dendritic Cells Prevents Excessive T Cell Priming by Silencing Endogenous Retrovirus.

Journal of immunology (Baltimore, Md. : 1950)

Chikuma S,Yamanaka S,Nakagawa S,Ueda MT,Hayabuchi H,Tokifuji Y,Kanayama M,Okamura T,Arase H,Yoshimura A

Thu Apr 01 00:00:00 EDT 2021

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

Proceedings of the National Academy of Sciences of the United States of America

Li M,Xu X,Chang CW,Liu Y

Tue Sep 22 00:00:00 EDT 2020

TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis.

Science bulletin

Cui J,Hu J,Ye Z,Fan Y,Li Y,Wang G,Wang L,Wang Z

Tue Jul 30 00:00:00 EDT 2019

TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis.

Science bulletin

Cui J,Hu J,Ye Z,Fan Y,Li Y,Wang G,Wang L,Wang Z

Tue Jul 30 00:00:00 EDT 2019

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

eLife

Ma X,Yang T,Luo Y,Wu L,Jiang Y,Song Z,Pan T,Liu B,Liu G,Liu J,Yu F,He Z,Zhang W,Yang J,Liang L,Guan Y,Zhang X,Li L,Cai W,Tang X,Gao S,Deng K,Zhang H

Thu Jan 17 00:00:00 EST 2019

TRIM28 promotes cervical cancer growth through the mTOR signaling pathway.

Oncology reports

Li F,Wang Z,Lu G

Sun Apr 01 00:00:00 EDT 2018

TRIM28 promotes cervical cancer growth through the mTOR signaling pathway.

Oncology reports

Li F,Wang Z,Lu G

Sun Apr 01 00:00:00 EDT 2018

ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.

DNA repair

Liu R,King A,Hoch NC,Chang C,Kelly GL,Deans AJ,Heierhorst J

Fri Sep 01 00:00:00 EDT 2017

ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.

DNA repair

Liu R,King A,Hoch NC,Chang C,Kelly GL,Deans AJ,Heierhorst J

Fri Sep 01 00:00:00 EDT 2017

TRIM28 knockdown increases sensitivity to etoposide by upregulating E2F1 in non-small cell lung cancer.

Oncology reports

Liu L,Xiao L,Liang X,Chen L,Cheng L,Zhang L,Wu X,Xu Q,Ma C

Thu Jun 01 00:00:00 EDT 2017

TRIM28 knockdown increases sensitivity to etoposide by upregulating E2F1 in non-small cell lung cancer.

Oncology reports

Liu L,Xiao L,Liang X,Chen L,Cheng L,Zhang L,Wu X,Xu Q,Ma C

Thu Jun 01 00:00:00 EDT 2017

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Cancer epidemiology

Liu L,Zhao E,Li C,Huang L,Xiao L,Cheng L,Huang X,Song Y,Xu D

Fri Feb 01 00:00:00 EST 2013

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Cancer epidemiology

Liu L,Zhao E,Li C,Huang L,Xiao L,Cheng L,Huang X,Song Y,Xu D

Fri Feb 01 00:00:00 EST 2013

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Cancer epidemiology

Liu L,Zhao E,Li C,Huang L,Xiao L,Cheng L,Huang X,Song Y,Xu D

Fri Feb 01 00:00:00 EST 2013

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Cancer epidemiology

Liu L,Zhao E,Li C,Huang L,Xiao L,Cheng L,Huang X,Song Y,Xu D

Fri Feb 01 00:00:00 EST 2013

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Hepatology (Baltimore, Md.)

Bojkowska K,Aloisio F,Cassano M,Kapopoulou A,Santoni de Sio F,Zangger N,Offner S,Cartoni C,Thomas C,Quenneville S,Johnsson K,Trono D

Mon Oct 01 00:00:00 EDT 2012

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Hepatology (Baltimore, Md.)

Bojkowska K,Aloisio F,Cassano M,Kapopoulou A,Santoni de Sio F,Zangger N,Offner S,Cartoni C,Thomas C,Quenneville S,Johnsson K,Trono D

Mon Oct 01 00:00:00 EDT 2012

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Hepatology (Baltimore, Md.)

Bojkowska K,Aloisio F,Cassano M,Kapopoulou A,Santoni de Sio F,Zangger N,Offner S,Cartoni C,Thomas C,Quenneville S,Johnsson K,Trono D

Mon Oct 01 00:00:00 EDT 2012

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Hepatology (Baltimore, Md.)

Bojkowska K,Aloisio F,Cassano M,Kapopoulou A,Santoni de Sio F,Zangger N,Offner S,Cartoni C,Thomas C,Quenneville S,Johnsson K,Trono D

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28

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Gene Aliases: AA408787; KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; Tif1beta; TRIM28

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UniProt ID: (Human) Q13263, (Mouse) Q62318, (Rat) O08629

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Entrez Gene ID: (Human) 10155, (Mouse) 21849, (Rat) 116698

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Function(s)

RNA polymerase II transcription coactivator activity DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein kinase activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase binding Krueppel-associated box domain binding sequence-specific DNA binding poly(A) RNA binding chromo shadow domain binding promoter-specific chromatin binding transcription coactivator activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development epithelial to mesenchymal transition DNA repair transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation embryo implantation protein ubiquitination protein sumoylation positive regulation of transcription factor import into nucleus DNA methylation involved in embryo development innate immune response positive regulation of DNA repair negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated protein autophosphorylation protein oligomerization convergent extension involved in axis elongation embryonic placenta morphogenesis negative regulation of DNA demethylation negative regulation of viral release from host cell regulation of genetic imprinting transcription initiation from RNA polymerase II promoter Ras protein signal transduction covalent chromatin modification positive regulation of transcription from RNA polymerase II promoter positive regulation of methylation-dependent chromatin silencing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KAP1 Polyclonal Antibody

KAP1 Antibody (15202-1-AP) in ICC/IF

Product Details

15202-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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