Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
KCNK18 (TRESK) (extracellular) Antibody (APC-122-200UL) in ICC/IF
Expression of KCNK18 in ND7/23 cell line - Cell surface detection of KCNK18 in living mouse/rat neuroblastoma x dorsal root ganglion neuron hybrid cell line (ND7/23). A. Extracellular staining of cells with Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:50) followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (bl... View More
Product Details
APC-122-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18, 1st extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
TRESK (TWIK-related spinal cord K+ channel) is a two-pore domain K+ channel that induces outward rectification and functions as a background K+ channel that is abundantly expressed in the spinal cord, thymus, spleen, and leukemic T-lymphocytes. TRESK is activated by increased cytoplasmic calcium concentration through calcineurin as well as by acetylcholine and histamine. It has been suggested that TRESK is linked to acute and chronic pain by activation of calcineurin. Other studies indicate that TRESK may play a role in the regulation of T-cell function and could be an excellent target to treat T-cell originated immune dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 2P K ion channel TRESK; Potassium channel subfamily K member 18; potassium channel, subfamily K, member 18; tandem-pore K+ (K(2P)) channel; Tresk-2; TWIK-related individual K+ channel; TWIK-related spinal cord K+ channel; Two-pore-domain potassium channel TRESK
Gene Aliases: Gm781; Kcnk18; Tresk; Tresk-2; Tresk2; Trik
UniProt ID: (Rat) Q6Q1P3, (Mouse) Q6VV64
Entrez Gene ID: (Rat) 445371, (Mouse) 332396
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support