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KCNN3 (KCa2.3, SK3) Antibody (APC-025-200UL) in IHC
Expression of KCa2.3 (SK3) in mouse dopaminergic neurons - Immunohistochemical staining ofmouse dopaminergic neurons using Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025). A. KCa2.3 is detectedinsubstantia nigra pars compacta. B. Tyrosine hydroxylase staining shows dopaminergic neurons. Triangles point at cells with co-localization.
Product Details
APC-025-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3, Intracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. KCNN3, a member of the KCNN family of potassium channel genes, encodes a protein that is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KCa2.3; potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 3; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3; SK3; SKCa 3; SKCa3; small conductance calcium-activated potassium channel 3; Small conductance calcium-activated potassium channel protein 3; small-conductance Ca2+-activated K+ channel 3
Gene Aliases: hSK3; K3; KCa2.3; KCNN3; SK3; SKCA3
UniProt ID: (Human) Q9UGI6, (Mouse) P58391, (Rat) P70605
Entrez Gene ID: (Human) 3782, (Mouse) 140493, (Rat) 54263
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