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Invitrogen
KEAP1 Recombinant Rabbit Monoclonal Antibody (PSH0-90)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
KEAP1 Antibody (MA5-49609) in IHC (P)
Immunohistochemical analysis of KEAP1 on formalin-fixed paraffin-embedded mouse kidney tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with KEAP1 Rabbit Recombinant Monoclonal Antibody (PSH0-90) (Product # MA5-49609) at 1:1,000 dilution for 1 hour at r... View More
Product Details
MA5-49609
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-90
Immunogen
Recombinant protein within human keap1 aa 275-624/624
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094136
Product Specific Information
Sequence Similarities: 98% Mouse/Rat.
Tissue Specificity: Broadly expressed, with highest levels in skeletal muscle.
Positive Control: HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, A431 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Daudi cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue, rat kidney tissue, PC-12.
Subcellular Location: Cytoplasm, Nucleus.
Predicted band size: 70 kDa.
Target Information
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytosolic inhibitor of Nrf2; INrf2; Kelch-like ECH-associated protein 1; kelch-like family member 19; Kelch-like protein 19; NRF2 cytosolic inhibitor; ring canal protein
Gene Aliases: INRF2; KEAP1; KIAA0132; KLHL19; mKIAA0132
UniProt ID: (Human) Q14145, (Mouse) Q9Z2X8
Entrez Gene ID: (Human) 9817, (Mouse) 50868, (Rat) 117519
in utero embryonic development
selenium compound metabolic process
malate metabolic process
transcription, DNA-templated
flavonoid metabolic process
response to metal ion
proteasomal ubiquitin-independent protein catabolic process
negative regulation of gene expression
protein ubiquitination
alkanesulfonate metabolic process
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to immobilization stress
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
cytoplasmic sequestering of transcription factor
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of epidermal cell differentiation
protein oligomerization
cellular response to interleukin-4
cellular response to organic cyclic compound
response to thyroid hormone
regulation of transcription, DNA-templated
positive regulation of cellular protein metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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