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KLF4 Antibody (710659) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HEK 293 cells for detection of KLF4 using Anti-KLF4 Recombinant Rabbit Polyclonal Antibody (Product # 710659, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of KLF4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, ... View More
Product Details
710659
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
1HCLC
Immunogen
full-length recombinant protein corresponding to human KLF4 (aa 1-118)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532749
Product Specific Information
This antibody is predicted to react with Monkey, Pig and Rabbit.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: endothelial Kruppel-like zinc finger protein; Epithelial zinc finger protein EZF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (gut); RP11-150J11.1
Gene Aliases: EZF; GKLF; KLF4
UniProt ID: (Human) O43474
Entrez Gene ID: (Human) 9314
core promoter proximal region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding transcription factor recruiting
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription cofactor binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
zinc ion binding
phosphatidylinositol 3-kinase regulator activity
histone deacetylase binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
mesodermal cell fate determination
negative regulation of cell proliferation
epidermal cell differentiation
positive regulation of gene expression
negative regulation of gene expression
negative regulation of phosphatidylinositol 3-kinase signaling
negative regulation of muscle hyperplasia
stem cell population maintenance
post-embryonic camera-type eye development
negative regulation of NF-kappaB transcription factor activity
positive regulation of cellular protein metabolic process
negative regulation of heterotypic cell-cell adhesion
somatic stem cell population maintenance
post-embryonic hemopoiesis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of phosphatidylinositol 3-kinase activity
negative regulation of interleukin-8 biosynthetic process
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hemoglobin biosynthetic process
negative regulation of smooth muscle cell proliferation
regulation of axon regeneration
epidermis morphogenesis
negative regulation of inflammatory response
positive regulation of protein metabolic process
negative regulation of protein kinase B signaling
positive regulation of telomerase activity
canonical Wnt signaling pathway
negative regulation of response to cytokine stimulus
cellular response to hydrogen peroxide
negative regulation of ERK1 and ERK2 cascade
cellular response to retinoic acid
cellular response to growth factor stimulus
cellular response to cycloheximide
cellular response to laminar fluid shear stress
negative regulation of cell migration involved in sprouting angiogenesis
cellular response to peptide
negative regulation of leukocyte adhesion to arterial endothelial cell
negative regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of transcription regulatory region DNA binding
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