Bethyl Laboratories

KLF4 Recombinant Rabbit Monoclonal Antibody (BLR250L)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

A700-250

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

6 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR250L

Immunogen

Peptide representing a region between residue 75 and 125 of Human Krueppel-like factor 4.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

BBS, pH 8.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Target Information

Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endothelial Kruppel-like zinc finger protein; Epithelial zinc finger protein EZF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF); Kruppel-like factor 4 (gut); RP11-150J11.1

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Gene Aliases: EZF; GKLF; KLF4; Zie

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UniProt ID: (Human) O43474, (Mouse) Q60793

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Entrez Gene ID: (Human) 9314, (Mouse) 16600

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Function(s)
core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor recruiting core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription cofactor binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding zinc ion binding phosphatidylinositol 3-kinase regulator activity histone deacetylase binding transcription regulatory region DNA binding nucleic acid binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding metal ion binding C2H2 zinc finger transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter mesodermal cell fate determination negative regulation of cell proliferation epidermal cell differentiation positive regulation of gene expression negative regulation of gene expression negative regulation of phosphatidylinositol 3-kinase signaling negative regulation of muscle hyperplasia stem cell population maintenance post-embryonic camera-type eye development negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process negative regulation of heterotypic cell-cell adhesion somatic stem cell population maintenance post-embryonic hemopoiesis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process fat cell differentiation regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hemoglobin biosynthetic process negative regulation of smooth muscle cell proliferation regulation of axon regeneration epidermis morphogenesis negative regulation of inflammatory response positive regulation of protein metabolic process negative regulation of protein kinase B signaling positive regulation of telomerase activity canonical Wnt signaling pathway negative regulation of response to cytokine stimulus cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to growth factor stimulus cellular response to cycloheximide cellular response to laminar fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis cellular response to peptide negative regulation of leukocyte adhesion to arterial endothelial cell negative regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of transcription regulatory region DNA binding transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to organic substance cell differentiation response to retinoic acid regulation of cell proliferation
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