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KPNB1 Antibody (A300-482A) in WB
Detection of KPNB1 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (50 µg) from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-KPNB1 antibody A300-482A used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). immunoprecipitation of KPNB1 was unsuccessful when using rabbit anti-KPNB1 antibody A300-481A at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 s... View More
Product Details
A300-482A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 825 and the C-terminus (residue 876) of human Karyopherin (importin) beta 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:6400; importin 1; importin 90; importin beta; importin beta-1 subunit; Importin subunit beta-1; Importin-90; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; Karyopherin subunit beta-1; MGC2155; MGC2156; MGC2157; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG
Gene Aliases: AA409963; IMB1; Impnb; IPO1; IPOB; KPNB1; NTF97
UniProt ID: (Human) Q14974, (Mouse) P70168
Entrez Gene ID: (Human) 3837, (Mouse) 16211
protein import into nucleus, docking
protein import into nucleus, translocation
apoptotic DNA fragmentation
protein import into nucleus
NLS-bearing protein import into nucleus
ribosomal protein import into nucleus
mitotic chromosome movement towards spindle pole
mitotic metaphase plate congression
modulation by virus of host process
astral microtubule organization
Ran protein signal transduction
establishment of mitotic spindle localization
establishment of protein localization
intracellular transport of virus
mitotic spindle assembly
transport
intracellular protein transport
nucleocytoplasmic transport
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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