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Invitrogen
Kappa Light Chain Monoclonal Antibody (TB28-2), PE-Cyanine7, eBioscience™
FIGURE: 1 / 1
Kappa Light Chain Antibody (25-9970-42) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0119-42) and Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (left) or Ig kappa light chain Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
25-9970-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TB28-2
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802271
Product Specific Information
Description: The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin. Approximately 22-kDa, the Ig kappa chain is comprised of one variable and one constant region. Two kappa chains together with the heavy chain comprise the whole Ig structure. Expression of Ig kappa is typically found on more than half of B lymphocytes.
Applications Reported: This TB28-2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TB28-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ig kappa chain c region; Immunoglobulin K; immunoglobulin kappa light chain; Kappa LC
Gene Aliases: HCAK1; IGK@; IGKCD; Km
Entrez Gene ID: (Human) 50802, (Human) 3514
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