Invitrogen

Klotho Polyclonal Antibody

4 Published Figures

8 References

View all (15) Klotho antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (4)

Klotho Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

Klotho Antibody (PA5-21078) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Klotho Polyclonal Antibody (Product # PA5-21078) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary... View More

Klotho Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Klotho Antibody in Immunocytochemistry (ICC/IF)

Klotho Antibody in Western Blot, Neutralization (WB, Neu)

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Product Details

PA5-21078

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 17 amino acid peptide near the center of human KLOTHO.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153007

Product Specific Information

A suggested positive control is HepG2 cell lysate.

PA5-21078 can be used with blocking peptide PEP-1192.

Target Information

KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Trade-offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model.

Toxicological research

Solek P,Mytych J,Sujkowska E,Grzegorczyk M,Jasiewicz P,Sowa-Kucma M,Stachowicz K,Koziorowski M,Tabecka-Lonczynska A

PA5-21078 was used in Western Blotting to suggest that the tested ALS and LPS can contribute to a reduction of male fertility, but NS-398, IMI, and IMI+NS-398 may also act as stimulants after LPS.

Fri Apr 01 00:00:00 EDT 2022

Fibroblast growth factor-23 and parathyroid hormone suppress small intestinal magnesium absorption.

Physiological reports

Suksridechacin N,Thongon N

PA5-21078 was used in Western Blotting to propose a novel magnesiotropic action of PTH and FGF-23 by modulating small intestinal Mg2+ absorption.

Fri Apr 01 00:00:00 EDT 2022

FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling.

Cell death & disease

Chang HM,Peng KY,Chan CK,Sun CY,Chen YY,Chang HM,Huang CL,Liu PC,Chen PY,Wang KC,Wang WJ,Wu CC,Lin YF,Lai TS,Huang TM,Young GH,Lin SL,Ostermann M,Chu TS,Chueh JS,Wu VC

PA5-21078 was used in Western Blot to highlight a revolutionary and important role that FGF23 plays in the nephroprotection of IR-AKI.

Sat Apr 17 00:00:00 EDT 2021

FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling.

Cell death & disease

Chang HM,Peng KY,Chan CK,Sun CY,Chen YY,Chang HM,Huang CL,Liu PC,Chen PY,Wang KC,Wang WJ,Wu CC,Lin YF,Lai TS,Huang TM,Young GH,Lin SL,Ostermann M,Chu TS,Chueh JS,Wu VC

PA5-21078 was used in Western Blot to highlight a revolutionary and important role that FGF23 plays in the nephroprotection of IR-AKI.

Sat Apr 17 00:00:00 EDT 2021

FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling.

Cell death & disease

Chang HM,Peng KY,Chan CK,Sun CY,Chen YY,Chang HM,Huang CL,Liu PC,Chen PY,Wang KC,Wang WJ,Wu CC,Lin YF,Lai TS,Huang TM,Young GH,Lin SL,Ostermann M,Chu TS,Chueh JS,Wu VC

PA5-21078 was used in Western Blot to highlight a revolutionary and important role that FGF23 plays in the nephroprotection of IR-AKI.

Sat Apr 17 00:00:00 EDT 2021

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA5-21078 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

PA5-21078 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

microRNA-200c regulates KLOTHO expression in human kidney cells under oxidative stress.

PloS one

Morii K,Yamasaki S,Doi S,Irifuku T,Sasaki K,Doi T,Nakashima A,Arihiro K,Masaki T

PA5-21078 was used in Immunocytochemistry-immunoflourescence to study KLOTHO expression and its regulation by miR-200c.

Fri Feb 14 00:00:00 EST 2020

The Role of the Anti-Aging Protein Klotho in IGF-1 Signaling and Reticular Calcium Leak: Impact on the Chemosensitivity of Dedifferentiated Liposarcomas.

Cancers

Delcroix V,Mauduit O,Tessier N,Montillaud A,Lesluyes T,Ducret T,Chibon F,Van Coppenolle F,Ducreux S,Vacher P

PA5-21078 was used in Western Blotting to provide a new therapeutic strategy in the management of DDLPS.

Wed Nov 14 00:00:00 EST 2018

Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.

Experimental cell research

Mytych J,Wos I,Solek P,Koziorowski M

PA521078 was used in immunocytochemistry and western blot to show that ROS-induced DNA damage reduces metabolic activity, inhibits proliferation, and induces cell cycle arrest followed by activation of p16-, p21-, and p27- mediated DNA damage response pathways, premature senescence, and apoptosis induction

Sun Jan 15 00:00:00 EST 2017

Bioinformatics

Protein Aliases: HFTC3; Klotho; secreted form of Klotho protein

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Gene Aliases: alpha-kl; KL

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UniProt ID: (Human) Q9UEF7, (Rat) Q9Z2Y9, (Mouse) O35082

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Entrez Gene ID: (Human) 9365, (Rat) 83504, (Mouse) 16591

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Function(s)

beta-glucuronidase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding hormone activity vitamin D binding beta-glucosidase activity 1-phosphatidylinositol-3-kinase activity fibroblast growth factor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity hydrolase activity, hydrolyzing O-glycosyl compounds protein binding hydrolase activity hydrolase activity, acting on glycosyl bonds glycosidase

Process(es)

MAPK cascade carbohydrate metabolic process energy reserve metabolic process aging insulin receptor signaling pathway fibroblast growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling positive regulation of bone mineralization phosphatidylinositol-3-phosphate biosynthetic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling calcium ion homeostasis positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway glycosyl compound metabolic process acute inflammatory response metabolic process

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Klotho Polyclonal Antibody

Klotho Antibody (PA5-21078) in IHC (P)

Product Details

PA5-21078

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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