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LAT Antibody (A305-893A) in WB
Detection of human LAT by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LAT antibody (A305-893A lot 1) used for WB at 0.04 mg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-893A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 212 and 262 of human Linker for activation of T-cells family member 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate, tris citrate
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Predicted reactivity by homology: Rat, Mouse.
Target Information
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36
Gene Aliases: LAT; LAT1; pp36
UniProt ID: (Human) O43561
Entrez Gene ID: (Human) 27040
MAPK cascade
adaptive immune response
lymphocyte homeostasis
inflammatory response
immune response
integrin-mediated signaling pathway
Ras protein signal transduction
positive regulation of signal transduction
gene expression
calcium-mediated signaling
platelet activation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
mast cell degranulation
positive regulation of GTPase activity
positive regulation of protein kinase activity
T cell receptor signaling pathway
regulation of T cell activation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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