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LAT Antibody (PA5-120935) in WB
Western blot analysis of LAT in extracts of THP-1 cells. Samples were incubated with LAT Polyclonal antibody (Product # PA5-120935) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120935
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 95-262 of human LAT (NP_055202.1).
Conjugate
Form
Liquid
Concentration
1.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914507
Product Specific Information
Positive test controls include: THP-1. The target is usually found in the following locations: Cell membrane, Single-pass type III membrane protein.
Immunogen sequence: PSSRRDSDGA NSVASYENEG ASGIRGAQAG WGVWGPSWTR LTPVSLPPEP ACEDADEDED DYHNPGYLVV LPDSTPATST AAPSAPALST PGIRDSAFSM ESIDDYVNVP ESGESAEASL DGSREYVNVS QELHPGAAKT EPAALSSQEA EEVEEEGAPD YENLQELN
Target Information
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36
Gene Aliases: LAT; LAT1; pp36
UniProt ID: (Human) O43561
Entrez Gene ID: (Human) 27040
MAPK cascade
adaptive immune response
lymphocyte homeostasis
inflammatory response
immune response
integrin-mediated signaling pathway
Ras protein signal transduction
positive regulation of signal transduction
gene expression
calcium-mediated signaling
platelet activation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
mast cell degranulation
positive regulation of GTPase activity
positive regulation of protein kinase activity
T cell receptor signaling pathway
regulation of T cell activation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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