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LATS1 Antibody (MA5-51398) in WB
Western blot analysis of LATS1 in SH-SY5Y. Samples were incubated with LATS1 Recombinant monoclonal antibody (Product # MA5-51398) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-51398
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5I10Q5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LATS1 (NP_004681.1).
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093084
Product Specific Information
Immunogen sequence: MKRSEKPEGY RQMRPKTFPA SNYTVSSRQM LQEIRESLRN LSKPSDAAKA EHNMSKMSTE DPRQVRNPPK FGTHHKALQE IRNSLLPFAN ETNSSRSTSE
Target Information
LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis which is phosphorylated in a cell-cycle dependent manner. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity which indicating a role as a negative regulator of CDC2/cyclin A and the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. LATS1 act as a tumor suppressor and play an important role in the development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-warts; Large tumor suppressor homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS, large tumor suppressor, homolog 1; LATS1; Serine/threonine-protein kinase LATS1; WARTS protein kinase
Gene Aliases: LATS1; WARTS; wts
UniProt ID: (Human) O95835
Entrez Gene ID: (Human) 9113
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
sister chromatid segregation
inner cell mass cell fate commitment
inner cell mass cellular morphogenesis
protein phosphorylation
mitotic nuclear division
hormone-mediated signaling pathway
keratinocyte differentiation
regulation of actin filament polymerization
positive regulation of peptidyl-serine phosphorylation
hippo signaling
positive regulation of apoptotic process
regulation of protein complex assembly
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of organ growth
cytoplasmic sequestering of protein
cell division
negative regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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