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Product Details
24497-1-AP
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
LCK Fusion Protein Ag21475 (1-539 aa encoded by BC013200)
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MGCGCSSHP EDDWMENIDV CENCHYPIVP LDGKGTLLIR NGSEVRDPLV TYEGSNPPAS PLQDNLVIAL HSYEPSHDGD LGFEKGEQLR ILEQSGEWWK AQSLTTGQEG FIPFNFVAKA NSLEPEPWFF KNLSRKDAER QLLAPGNTHG SFLIRESEST AGSFSLSVRD FDQNQGEVVK HYKIRNLDNG GFYISPRITF PGLHELVRHY TNASDGLCTR LSRPCQTQKP QKPWWEDEWE VPRETLKLVE RLGAGQFGEV WMGYYNGHTK VAVKSLKQGS MSPDAFLAEA NLMKQLQHQR LVRLYAVVTQ EPIYIITEYM ENDTLLDSQL EEKGLGASPW GNLGQQLLLL PTGSLVDFLK TPSGIKLTIN KLLDMAAQIA EGMAFIEERN YIHRDLRAAN ILVSDTLSCK IADFGLARLI EDNEYTAREG AKFPIKWTAP EAINYGTFTI KSDVWSFGIL LTEIVTHGRI PYPGMTNPEV IQNLERGYRM VRPDNCPEEL YQLMRLCWKE RPEDRPTFDY LRSVLEDFFT ATEGQYQPQP (1-539 aa encoded by BC013200)
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
Gene Aliases:
Hck-3; IMD22; LCK; LSK; Lsk-t; Lskt; p56
UniProt ID: (Human) P06239, (Mouse) P06240
Entrez Gene ID: (Human) 3932, (Mouse) 16818
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
ATPase binding
nucleotide binding
antigen binding
protein kinase activity
receptor binding
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
protein phosphorylation
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
response to mechanical stimulus
response to metal ion
positive regulation of gene expression
phosphorylation
dephosphorylation
peptidyl-tyrosine phosphorylation
T cell differentiation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
response to hydrogen peroxide
regulation of apoptotic process
innate immune response
positive regulation of gamma-delta T cell differentiation
protein autophosphorylation
B cell receptor signaling pathway
regulation of T cell receptor signaling pathway
positive regulation of T cell activation
release of sequestered calcium ion into cytosol
positive regulation of uterine smooth muscle contraction
cellular response to peptide hormone stimulus
positive regulation of intrinsic apoptotic signaling pathway
protein dephosphorylation
cellular zinc ion homeostasis
regulation of phosphatidylinositol 3-kinase signaling
viral process
hemopoiesis
platelet activation
T cell costimulation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of defense response to virus by virus
T cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
leukocyte migration
regulation of lymphocyte activation
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