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FIGURE: 1 / 3
LCK Antibody (H00003932-D01P) in WB
Western Blot analysis of LCK expression in transfected 293T cell line (H00003932-T02) by LCK MaxPab polyclonal antibody.
Lane 1: LCK transfected lysate(61.20 KDa).
Lane 2: Non-transfected lysate.
Lane 1: LCK transfected lysate(61.20 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00003932-D01P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
LCK (AAH13200.1, 1 a.a. approximately 539 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MGCGCSSHPE DDWMENIDVC ENCHYPIVPL DGKGTLLIRN GSEVRDPLVT YEGSNPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP GLHELVRHYT NASDGLCTRL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHQRL VRLYAVVTQE PIYIITEYME NDTLLDSQLE EKGLGASPWG NLGQQLLLLP TGSLVDFLKT PSGIKLTINK LLDMAAQIAE GMAFIEERNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI QNLERGYRMV RPDNCPEELY QLMRLCWKER PEDRPTFDYL RSVLEDFFTA TEGQYQPQP
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
Gene Aliases: IMD22; LCK; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
ATPase binding
protein phosphorylation
protein dephosphorylation
cellular zinc ion homeostasis
activation of cysteine-type endopeptidase activity involved in apoptotic process
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
viral process
hemopoiesis
platelet activation
T cell differentiation
T cell costimulation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
response to drug
innate immune response
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of defense response to virus by virus
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
leukocyte migration
release of sequestered calcium ion into cytosol
regulation of lymphocyte activation
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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