Proteintech

LDHA Polyclonal Antibody

LDHA Antibody in Immunocytochemistry (ICC/IF)

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LDHA Antibody (21799-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 21799-1-AP (LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21799-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:30,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LDHA Fusion Protein Ag16703 (283-332 aa encoded by BC067223)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IKDDVFLSV PCILGQNGIS DLVKVTLTSE EEARLKKSAD TLWGIQKELQ F (283-332 aa encoded by BC067223)

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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LDHA is a prognostic biomarker on the immune response in pancreatic adenocarcinoma and associated with m6A modification.

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PNAS nexus

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The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Molecular cell

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Thu Feb 03 00:00:00 EST 2022

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Molecular cell

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Thu Feb 03 00:00:00 EST 2022

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Molecular cell

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Thu Feb 03 00:00:00 EST 2022

The Mixture of Ferulic Acid and P-Coumaric Acid Suppresses Colorectal Cancer through lncRNA 495810/PKM2 Mediated Aerobic Glycolysis.

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CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism.

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CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism.

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Zhu B,Cao X,Zhang W,Pan G,Yi Q,Zhong W,Yan D

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Zhu B,Cao X,Zhang W,Pan G,Yi Q,Zhong W,Yan D

Tue Jan 01 00:00:00 EST 2019

MicroRNA-31-5p enhances the Warburg effect via targeting FIH.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhu B,Cao X,Zhang W,Pan G,Yi Q,Zhong W,Yan D

Tue Jan 01 00:00:00 EST 2019

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhu B,Cao X,Zhang W,Pan G,Yi Q,Zhong W,Yan D

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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

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American journal of translational research

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Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

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CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

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Menin enhances c-Myc-mediated transcription to promote cancer progression.

Nature communications

Wu G,Yuan M,Shen S,Ma X,Fang J,Zhu L,Sun L,Liu Z,He X,Huang D,Li T,Li C,Wu J,Hu X,Li Z,Song L,Qu K,Zhang H,Gao P

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HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.

Cancer research

Li Y,Wang Z,Shi H,Li H,Li L,Fang R,Cai X,Liu B,Zhang X,Ye L

Fri Jan 15 00:00:00 EST 2016

Hypoxia-induced regulation of placental REDD1 and mTOR was impaired in a rat model of estrogen-induced cholestasis.

Archives of gynecology and obstetrics

Zhou F,Chen H,Wang X,Yu P,Hu Y

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Hypoxia-induced regulation of placental REDD1 and mTOR was impaired in a rat model of estrogen-induced cholestasis.

Archives of gynecology and obstetrics

Zhou F,Chen H,Wang X,Yu P,Hu Y

Tue Nov 01 00:00:00 EDT 2016

Hypoxia-induced regulation of placental REDD1 and mTOR was impaired in a rat model of estrogen-induced cholestasis.

Archives of gynecology and obstetrics

Zhou F,Chen H,Wang X,Yu P,Hu Y

Tue Nov 01 00:00:00 EDT 2016

Hypoxia-induced regulation of placental REDD1 and mTOR was impaired in a rat model of estrogen-induced cholestasis.

Archives of gynecology and obstetrics

Zhou F,Chen H,Wang X,Yu P,Hu Y

Tue Nov 01 00:00:00 EDT 2016

Expression of hypoxia-regulated genes and glycometabolic genes in placenta from patients with intrahepatic cholestasis of pregnancy.

Placenta

Wei W,Hu YY

Mon Sep 01 00:00:00 EDT 2014

Expression of hypoxia-regulated genes and glycometabolic genes in placenta from patients with intrahepatic cholestasis of pregnancy.

Placenta

Wei W,Hu YY

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; M-LDH; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

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Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; Ldh3; LDHA; LDHM; PIG19

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UniProt ID: (Human) P00338, (Rat) P04642, (Mouse) P06151

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Entrez Gene ID: (Human) 3939, (Rat) 24533, (Mouse) 16828, (Rat) 29634

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Function(s)

L-lactate dehydrogenase activity protein binding kinase binding identical protein binding NAD binding cadherin binding involved in cell-cell adhesion lactate dehydrogenase activity catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor dehydrogenase

Process(es)

response to hypoxia lactate metabolic process pyruvate metabolic process glycolytic process response to nutrient response to glucose NAD metabolic process response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen post-embryonic organ development response to cAMP oxidation-reduction process cell-cell adhesion response to organic cyclic compound substantia nigra development cellular response to extracellular stimulus cellular carbohydrate metabolic process carbohydrate metabolic process lactate biosynthetic process from pyruvate carboxylic acid metabolic process ATP biosynthetic process lactate oxidation sperm motility

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LDHA Polyclonal Antibody

LDHA Antibody (21799-1-AP) in ICC/IF

Product Details

21799-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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