Invitrogen

LDHA Polyclonal Antibody

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

7 Published Figures

11 References

View all (59) LDHA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

LDHA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

LDHA Antibody (PA5-27406) in ICC/IF

Immunofluorescence analysis of LDHA was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with LDHA Polyclonal Antibody (Product # PA5-27406) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More

LDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LDHA Antibody in Western Blot, RNA Immunoprecipitation (WB, RIP)

Product Image Gallery

LDHA Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Western blot of LDHA using LDHA Polyclonal Antibody (Product # PA5-27406), tested in parallel with LDHA Polyclonal Antibody (Product # PA5-17183) and LDHA Polyclonal Antibody (Product # PA5-23036), shows similar expression of LDHA in the cell lines tested. {IAV}

Product Details

PA5-27406

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 133 and 332 of Human Lactate Dehydrogenase

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544882

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, MCF-7, MDA-MB-231, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (92%), Rat (91%), Xenopus laevis (80%), Pig (90%), Rabbit (91%), Chicken (80%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment.

Viruses

Harrison MAA,Hochreiner EM,Benjamin BP,Lawler SE,Zwezdaryk KJ

PA5-27406 was used in Western Blot to characterize the metabolic reprogramming that occurs following acute infection of glioblastoma with human herpesvirus-5 (cytomegalovirus) in vitro.

Fri Jan 07 00:00:00 EST 2022

Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment.

Viruses

Harrison MAA,Hochreiner EM,Benjamin BP,Lawler SE,Zwezdaryk KJ

PA5-27406 was used in Western Blot to characterize the metabolic reprogramming that occurs following acute infection of glioblastoma with human herpesvirus-5 (cytomegalovirus) in vitro.

Fri Jan 07 00:00:00 EST 2022

Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotection.

iScience

Xu B,Li F,Zhang W,Su Y,Tang L,Li P,Joshi J,Yang A,Li D,Wang Z,Wang S,Xie J,Gu H,Zhu W

PA5-27406 was used in Western Blotting to suggest that FGF1/CHIR99021 protects the heart against ischemic injury via improving myocardial metabolism which may be exploited for treatment of acute myocardial infarction in humans.

Fri Jun 17 00:00:00 EDT 2022

Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.

Science advances

Abu El Maaty MA,Terzic J,Keime C,Rovito D,Lutzing R,Yanushko D,Parisotto M,Grelet E,Namer IJ,Lindner V,Laverny G,Metzger D

PA5-27406 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to characterize prostatic intraepithelial neoplasia (PIN) progression and demonstrate that HIF1A is a target for PCa prevention and that TGM2 is a promising prognostic biomarker of early relapse after prostatectomy.

Fri Jul 22 00:00:00 EDT 2022

Metabolic Profile in Neonatal Pig Hearts.

Frontiers in cardiovascular medicine

Li P,Li F,Tang L,Zhang W,Jin Y,Gu H,Zhu W

PA5-27406 was used in Western Blotting to evaluate the metabolic profile in pig hearts at postnatal day 1, 3, 7, and 28 (P1, P3, P7, and P28, respectively).

Tue May 17 00:00:00 EDT 2022

Metabolic Profile in Neonatal Pig Hearts.

Frontiers in cardiovascular medicine

Li P,Li F,Tang L,Zhang W,Jin Y,Gu H,Zhu W

PA5-27406 was used in Western Blotting to evaluate the metabolic profile in pig hearts at postnatal day 1, 3, 7, and 28 (P1, P3, P7, and P28, respectively).

Tue May 17 00:00:00 EDT 2022

Lactate oxidative phosphorylation by annulus fibrosus cells: evidence for lactate-dependent metabolic symbiosis in intervertebral discs.

Arthritis research & therapy

Wang D,Hartman R,Han C,Zhou CM,Couch B,Malkamaki M,Roginskaya V,Van Houten B,Mullett SJ,Wendell SG,Jurczak MJ,Kang J,Lee J,Sowa G,Vo N

Published figure using LDHA polyclonal antibody (Product # PA5-27406) in Western Blot

Fri May 21 00:00:00 EDT 2021

SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells.

Cancers

D'Onofrio N,Martino E,Balestrieri A,Mele L,Neglia G,Balestrieri ML,Campanile G

Published figure using LDHA polyclonal antibody (Product # PA5-27406) in Western Blot

Sat Oct 16 00:00:00 EDT 2021

Lactate Reprograms Energy and Lipid Metabolism in Glucose-Deprived Oxidative Glioma Stem Cells.

Metabolites

Minami N,Tanaka K,Sasayama T,Kohmura E,Saya H,Sampetrean O

PA5-27406 was used in Western Blot to suggest that lactate is an important alternative energetic and biosynthetic substrate for oxidative GSCs, and that it sustains their growth under conditions of glucose deprivation.

Tue May 18 00:00:00 EDT 2021

Lactate Reprograms Energy and Lipid Metabolism in Glucose-Deprived Oxidative Glioma Stem Cells.

Metabolites

Minami N,Tanaka K,Sasayama T,Kohmura E,Saya H,Sampetrean O

Tue May 18 00:00:00 EDT 2021

LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.

Nature communications

Arra M,Swarnkar G,Ke K,Otero JE,Ying J,Duan X,Maruyama T,Rai MF,O'Keefe RJ,Mbalaviele G,Shen J,Abu-Amer Y

PA5-27406 was used in Western Blotting to investigate chondrocyte metabolism in the context of osteoarthritis.

Thu Jul 09 00:00:00 EDT 2020

LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.

Nature communications

Arra M,Swarnkar G,Ke K,Otero JE,Ying J,Duan X,Maruyama T,Rai MF,O'Keefe RJ,Mbalaviele G,Shen J,Abu-Amer Y

PA5-27406 was used in Western Blotting to investigate chondrocyte metabolism in the context of osteoarthritis.

Thu Jul 09 00:00:00 EDT 2020

LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.

Nature communications

Arra M,Swarnkar G,Ke K,Otero JE,Ying J,Duan X,Maruyama T,Rai MF,O'Keefe RJ,Mbalaviele G,Shen J,Abu-Amer Y

PA5-27406 was used in Western Blotting to investigate chondrocyte metabolism in the context of osteoarthritis.

Thu Jul 09 00:00:00 EDT 2020

p63 isoforms regulate metabolism of cancer stem cells.

Journal of proteome research

D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V

PA5-27406 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63

Fri Apr 04 00:00:00 EDT 2014

Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

Cell death & disease

Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A

PA5-27406 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells

Thu Jul 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; M-LDH; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

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Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; Ldh3; LDHA; LDHM; PIG19

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UniProt ID: (Human) P00338, (Rat) P04642, (Mouse) P06151

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Entrez Gene ID: (Human) 3939, (Rat) 24533, (Mouse) 16828, (Rat) 29634

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Function(s)

L-lactate dehydrogenase activity protein binding kinase binding identical protein binding NAD binding cadherin binding involved in cell-cell adhesion lactate dehydrogenase activity catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor dehydrogenase

Process(es)

response to hypoxia lactate metabolic process pyruvate metabolic process glycolytic process response to nutrient response to glucose NAD metabolic process response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen post-embryonic organ development response to cAMP oxidation-reduction process cell-cell adhesion response to organic cyclic compound substantia nigra development cellular response to extracellular stimulus cellular carbohydrate metabolic process carbohydrate metabolic process lactate biosynthetic process from pyruvate carboxylic acid metabolic process ATP biosynthetic process lactate oxidation sperm motility

Performance Guarantee

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LDHA Polyclonal Antibody

LDHA Antibody (PA5-27406) in ICC/IF

Product Details

PA5-27406

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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