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LDLR Antibody (MA5-29725) in ICC/IF
Immunofluorescence analysis of LDLR was performed using RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LDLR Rabbit Monoclonal Antibody (Product # MA5-29725) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27012) at a d... View More
Product Details
MA5-29725
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
032
Immunogen
Recombinant Mouse LDLR protein (Met1-Arg790)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785548
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse LDLR/LDL Receptor.
Target Information
The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LDL receptor; ldl-r; Low-density lipoprotein receptor
Gene Aliases: Hlb301; Ldlr
UniProt ID: (Mouse) P35951
Entrez Gene ID: (Mouse) 16835
lipid metabolic process
transport
lipid transport
endocytosis
receptor-mediated endocytosis
steroid metabolic process
cholesterol metabolic process
positive regulation of triglyceride biosynthetic process
regulation of phosphatidylcholine catabolic process
phospholipid transport
intestinal cholesterol absorption
cholesterol transport
low-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein catabolic process
cholesterol homeostasis
cholesterol import
regulation of cholesterol homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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