Proteintech

LEF1 Polyclonal Antibody

LEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

LEF1 Antibody (14972-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human thymus tissue slide using 14972-1-AP ( LEF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

LEF1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14972-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LEF1 Fusion Protein Ag6882 (1-399 aa encoded by BC050632)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPQLSGGGG GGGGDPELCA TDEMIPFKDE GDPQKEKIFA EISHPEEEGD LADIKSSLVN ESEIIPASNG HEVARQAQTS QEPYHDKARE HPDDGKHPDG GLYNKGPSYS SYSGYIMMPN MNNDPYMSNG SLSPPIPRTS NKVPVVQPSH AVHPLTPLIT YSDEHFSPGS HPSHIPSDVN SKQGMSRHPP APDIPTFYPL SPGGVGQITP PLGWQGQPVY PITGGFRQPY PSSLSVDTSM SRFSHHMIPG PPGPHTTGIP HPAIVTPQVK QEHPHTDSDL MHVKPQHEQR KEQEPKRPHI KKPLNAFMLY MKEMRANVVA ECTLKESAAI NQILGRRWHA LSREEQAKYY ELARKERQLH MQLYPGWSAR DNYGKKKKRK REKLQESASG TGPRMTAAYI (1-399 aa encoded by BC050632)

Target Information

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hordenine Activated Dermal Papilla Cells and Promoted Hair Regrowth by Activating Wnt Signaling Pathway.

Nutrients

Wang C,Zang K,Tang Z,Yang T,Ye X,Dang Y

Mon Jan 30 00:00:00 EST 2023

Combination of pioglitazone, a PPARγ agonist, and synthetic surfactant B-YL prevents hyperoxia-induced lung injury in adult mice lung explants.

Pulmonary pharmacology & therapeutics

Kurihara C,Sakurai R,Chuang TD,Waring AJ,Walther FJ,Rehan VK

Thu Jun 01 00:00:00 EDT 2023

Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity.

iScience

He T,Wang S,Li S,Shen H,Hou L,Liu Y,Wei Y,Xie F,Zhang Z,Zhao Z,Mo C,Guo H,Huang Q,Zhang R,Shen D,Li B

Fri Apr 21 00:00:00 EDT 2023

Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity.

iScience

He T,Wang S,Li S,Shen H,Hou L,Liu Y,Wei Y,Xie F,Zhang Z,Zhao Z,Mo C,Guo H,Huang Q,Zhang R,Shen D,Li B

Fri Apr 21 00:00:00 EDT 2023

Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity.

iScience

He T,Wang S,Li S,Shen H,Hou L,Liu Y,Wei Y,Xie F,Zhang Z,Zhao Z,Mo C,Guo H,Huang Q,Zhang R,Shen D,Li B

Fri Apr 21 00:00:00 EDT 2023

Ursolic Acid Suppresses Colorectal Cancer by Down-Regulation of Wnt/β-Catenin Signaling Pathway Activity.

Journal of agricultural and food chemistry

Zhao H,Tang S,Tao Q,Ming T,Lei J,Liang Y,Peng Y,Wang M,Liu M,Yang H,Ren S,Xu H

Wed Mar 08 00:00:00 EST 2023

Early growth response 2 in the mPFC regulates mouse social and cooperative behaviors.

Lab animal

Zhang Y,Feng W,Wang Z,Pang Y,Jin Y,Chen S,Ding S,Wang T,Zou Y,Sun P,Chen Y,Feng H,Huang H,Sheng C,Xiao M

Wed Feb 01 00:00:00 EST 2023

TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal.

Cellular & molecular immunology

Wang J,Zhao D,Lei Z,Ge P,Lu Z,Chai Q,Zhang Y,Qiang L,Yu Y,Zhang X,Li B,Zhu S,Zhang L,Liu CH

Wed Feb 01 00:00:00 EST 2023

YAP/TEAD1 and β-catenin/LEF1 synergistically induce estrogen receptor α to promote osteogenic differentiation of bone marrow stromal cells.

MedComm

Wang P,Huang L,Yang F,Chen W,Bai D,Guo Y

Thu Jun 01 00:00:00 EDT 2023

Exosomal miR-128-3p reversed fibrinogen-mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemia.

CNS neuroscience & therapeutics

Hou H,Wang Y,Yang L,Wang Y

Mon May 01 00:00:00 EDT 2023

Exosomal miRNA-181a-5p from the cells of the hair follicle dermal papilla promotes the hair follicle growth and development via the Wnt/β-catenin signaling pathway.

International journal of biological macromolecules

Zhao B,Li J,Zhang X,Dai Y,Yang N,Bao Z,Chen Y,Wu X

Sun May 15 00:00:00 EDT 2022

CYP2E1 plays a suppressive role in hepatocellular carcinoma by regulating Wnt/Dvl2/β-catenin signaling.

Journal of translational medicine

Zhu L,Yang X,Feng J,Mao J,Zhang Q,He M,Mi Y,Mei Y,Jin G,Zhang H

Wed May 04 00:00:00 EDT 2022

miR-145-5p targets MMP2 to protect brain injury in hypertensive intracerebral hemorrhage via inactivation of the Wnt/β-catenin signaling pathway.

Annals of translational medicine

Xiao W,Jiang Z,Wan W,Pan W,Xu J

Sun May 01 00:00:00 EDT 2022

Characterization and functional analysis of lncRNA2690 in regulating the growth cycle of the hair follicle in rabbits.

Animal genetics

Bao Z,Zhai P,Yao F,Ren Y,Zhao B,Chen Y,Wu X

Thu Dec 01 00:00:00 EST 2022

Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.

Evidence-based complementary and alternative medicine : eCAM

Yang X,Luo G,Fu L,Huang H,Wang L,Yin L,Zhang X,Wang T,Ma X,Feng T,Ye J

Sat Jul 16 00:00:00 EDT 2022

DNA Methylation Mediates lncRNA2919 Regulation of Hair Follicle Regeneration.

International journal of molecular sciences

Zhao B,Li J,Liu M,Yang N,Bao Z,Zhang X,Dai Y,Cai J,Chen Y,Wu X

Mon Aug 22 00:00:00 EDT 2022

SiNiSan alleviates liver injury by promoting hepatic stem cell differentiation via Wnt/β-catenin signaling pathway.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xu W,Du X,Li J,Zhang Z,Ma X,Luo D,Xiao M,Sun Q

Sun May 01 00:00:00 EDT 2022

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Cell death and differentiation

Hu L,Yin C,Chen D,Wu Z,Liang S,Zhang Y,Huang Z,Liu S,Xu X,Chen Z,Zhang Y,Qian A

Thu Jul 01 00:00:00 EDT 2021

Potential molecular mechanism of cardiac hypertrophy in mice induced by exposure to ambient PM_2.5.

Ecotoxicology and environmental safety

Liu Q,Han B,Zhang Y,Jiang T,Ning J,Kang A,Huang X,Zhang H,Pang Y,Zhang B,Wang Q,Niu Y,Zhang R

Thu Aug 19 00:00:00 EDT 2021

FHL1 Inhibits the Progression of Colorectal Cancer by Regulating the Wnt/β-Catenin Signaling Pathway.

Journal of Cancer

Liu Y,Wang C,Cheng P,Zhang S,Zhou W,Xu Y,Xu H,Ji G

Tue Aug 03 00:00:00 EDT 2021

Long non-coding RNA AC122108.1 promotes lung adenocarcinoma brain metastasis and progression through the Wnt/β-catenin pathway by directly binding to aldolase A.

Annals of translational medicine

Feng S,Liu H,Du P,Dong X,Pang Q,Guo H

Wed Dec 01 00:00:00 EST 2021

Phosphofructokinase-1 Inhibition Promotes Neuronal Differentiation of Neural Stem Cells and Functional Recovery After Stroke.

Neuroscience

Zhang F,Li Q,Liang H,Zhang Y

Thu Apr 01 00:00:00 EDT 2021

The protective mechanism of SIRT1 on cartilage through regulation of LEF-1.

BMC musculoskeletal disorders

Hu X,Feng G,Meng Z,Ma L,Jin Q

Tue Jul 27 00:00:00 EDT 2021

Comprehensive Analysis of the Potential Immune-Related Biomarker Transporter Associated With Antigen Processing 1 That Inhibits Metastasis and Invasion of Ovarian Cancer Cells.

Frontiers in molecular biosciences

Li X,Zeng S,Ding Y,Nie Y,Yang M

Mon Sep 19 00:00:00 EDT 2022

Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.

Frontiers in oncology

Shan MJ,Meng LB,Guo P,Zhang YM,Kong D,Liu YB

Tue Apr 26 00:00:00 EDT 2022

Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.

Frontiers in oncology

Shan MJ,Meng LB,Guo P,Zhang YM,Kong D,Liu YB

Tue Apr 26 00:00:00 EDT 2022

Stromal β-catenin activation impacts nephron progenitor differentiation in the developing kidney and may contribute to Wilms tumor.

Development (Cambridge, England)

Drake KA,Chaney CP,Das A,Roy P,Kwartler CS,Rakheja D,Carroll TJ

Fri Jul 31 00:00:00 EDT 2020

Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin.

Stem cells international

Wang Z,Jiang R,Wang L,Chen X,Xiang Y,Chen L,Xiao M,Ling L,Wang Y

Fri Apr 15 00:00:00 EDT 2022

Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.

The Journal of allergy and clinical immunology

Jing Y,Kang D,Liu L,Huang H,Chen A,Yang L,Jiang P,Li N,Miller H,Liu Z,Zhu X,Yang J,Wang X,Sun J,Liu Z,Liu W,Zhou X,Liu C

Fri Nov 01 00:00:00 EDT 2019

Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.

The Journal of allergy and clinical immunology

Jing Y,Kang D,Liu L,Huang H,Chen A,Yang L,Jiang P,Li N,Miller H,Liu Z,Zhu X,Yang J,Wang X,Sun J,Liu Z,Liu W,Zhou X,Liu C

Fri Nov 01 00:00:00 EDT 2019

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation.

Journal of cellular physiology

Yin C,Tian Y,Yu Y,Wang H,Wu Z,Huang Z,Zhang Y,Li D,Yang C,Wang X,Li Y,Qian A

Mon Jul 01 00:00:00 EDT 2019

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation.

Journal of cellular physiology

Yin C,Tian Y,Yu Y,Wang H,Wu Z,Huang Z,Zhang Y,Li D,Yang C,Wang X,Li Y,Qian A

Mon Jul 01 00:00:00 EDT 2019

AKIP1 promotes early recurrence of hepatocellular carcinoma through activating the Wnt/β-catenin/CBP signaling pathway.

Oncogene

Cui Y,Wu X,Lin C,Zhang X,Ye L,Ren L,Chen M,Yang M,Li Y,Li M,Li J,Guan J,Song L

Mon Jul 01 00:00:00 EDT 2019

Overexpression of MYC binding protein promotes invasion and migration in gastric cancer.

Oncology letters

Gong L,Xia Y,Qian Z,Shi J,Luo J,Song G,Xu J,Ye Z

Sun Apr 01 00:00:00 EDT 2018

Overexpression of MYC binding protein promotes invasion and migration in gastric cancer.

Oncology letters

Gong L,Xia Y,Qian Z,Shi J,Luo J,Song G,Xu J,Ye Z

Sun Apr 01 00:00:00 EDT 2018

Baicalin increases hair follicle development by increasing canonical Wnt/β‑catenin signaling and activating dermal papillar cells in mice.

International journal of molecular medicine

Xing F,Yi WJ,Miao F,Su MY,Lei TC

Sun Apr 01 00:00:00 EDT 2018

microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

He XY,Tan ZL,Mou Q,Liu FJ,Liu S,Yu CW,Zhu J,Lv LY,Zhang J,Wang S,Bao LM,Peng B,Zhao H,Zou L

Thu Jun 01 00:00:00 EDT 2017

microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

He XY,Tan ZL,Mou Q,Liu FJ,Liu S,Yu CW,Zhu J,Lv LY,Zhang J,Wang S,Bao LM,Peng B,Zhao H,Zou L

Thu Jun 01 00:00:00 EDT 2017

Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.

Oncotarget

Chen S,Sun YY,Zhang ZX,Li YH,Xu ZM,Fu WN

Tue Feb 28 00:00:00 EST 2017

Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.

Oncotarget

Chen S,Sun YY,Zhang ZX,Li YH,Xu ZM,Fu WN

Tue Feb 28 00:00:00 EST 2017

TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.

Cancer letters

Liu SL,Lin HX,Lin CY,Sun XQ,Ye LP,Qiu F,Wen W,Hua X,Wu XQ,Li J,Song LB,Guo L

Mon Aug 28 00:00:00 EDT 2017

Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis.

PloS one

Peng J,Lai ZG,Fang ZL,Xing S,Hui K,Hao C,Jin Q,Qi Z,Shen WJ,Dong QN,Bing ZH,Fu DL

Tue Dec 15 00:00:00 EST 2015

Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis.

PloS one

Peng J,Lai ZG,Fang ZL,Xing S,Hui K,Hao C,Jin Q,Qi Z,Shen WJ,Dong QN,Bing ZH,Fu DL

Tue Dec 15 00:00:00 EST 2015

Inhibition of phosphodiesterase 5 reduces bone mass by suppression of canonical Wnt signaling.

Cell death & disease

Gong Y,Xu CY,Wang JR,Hu XH,Hong D,Ji X,Shi W,Chen HX,Wang HB,Wu XM

Thu Nov 27 00:00:00 EST 2014

Inhibition of phosphodiesterase 5 reduces bone mass by suppression of canonical Wnt signaling.

Cell death & disease

Gong Y,Xu CY,Wang JR,Hu XH,Hong D,Ji X,Shi W,Chen HX,Wang HB,Wu XM

Thu Nov 27 00:00:00 EST 2014

Bioinformatics

Protein Aliases: DKFZp586H0919; FLJ46390; HGNC:6551; HMG Binding Domain; LEF-1; lymphoid enhancer binding factor 1 short isoform; Lymphoid enhancer-binding factor 1; OTTHUMP00000219736; OTTHUMP00000219737; T cell-specific transcription factor 1-alpha; TCF1-alpha

View more View less

Gene Aliases: 3000002B05; AI451430; LEF-1; LEF1; TCF10; TCF1ALPHA; TCF7L3

View more View less

UniProt ID: (Human) Q9UJU2, (Rat) Q9QXN1, (Mouse) P27782

View more View less

Entrez Gene ID: (Human) 51176, (Rat) 161452, (Mouse) 16842

View more View less

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding beta-catenin binding transcription factor binding DNA binding, bending estrogen receptor activity estrogen receptor binding enhancer binding histone binding histone deacetylase binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process sequence-specific DNA binding transcription regulatory region DNA binding gamma-catenin binding armadillo repeat domain binding C2H2 zinc finger domain binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

LEF1 Polyclonal Antibody

LEF1 Antibody (14972-1-AP) in IHC (P)

Product Details

14972-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!