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LEFTY2 Antibody (PA5-103061) in ICC/IF
Immunocytochemistry analysis of LEFTY2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with LEFTY2 polyclonal antibody (Product # PA5-103061) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103061
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LEFTY2(Accession O00292), corresponding to amino acid residues V203-P253.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852431
Product Specific Information
Antibody detects endogenous levels of total LEFTY2.
Target Information
Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: endometrial bleeding associated factor; Endometrial bleeding-associated factor; Left-right determination factor 2; Left-right determination factor A; Left-right determination factor B; left-right determination, factor A; MGC46222; Protein lefty-2; Protein lefty-A; Protein lefty-B; TGF-beta-4; Transforming growth factor beta-4
Gene Aliases: 6030463A22Rik; AV214969; EBAF; Ebaf2; LEFTA; Leftb; LEFTY2; LEFTYA; PSEC0024; TGFB4
UniProt ID: (Human) O00292, (Mouse) P57785
Entrez Gene ID: (Human) 7044, (Rat) 289316, (Mouse) 320202
platelet degranulation
transforming growth factor beta receptor signaling pathway
multicellular organism development
positive regulation of pathway-restricted SMAD protein phosphorylation
cell growth
regulation of apoptotic process
regulation of MAPK cascade
cell development
SMAD protein signal transduction
negative regulation of transcription from RNA polymerase II promoter
heart morphogenesis
determination of left/right symmetry
biological_process
negative regulation of cell proliferation
anterior/posterior axis specification
response to retinoic acid
cell migration involved in gastrulation
negative regulation of nodal receptor complex assembly
growth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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