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LGR5 Antibody (PA5-85589) in WB
Western blot analysis of LGR5 in non-transfected (-, unboiled) and LGR5-transfected (+, unboiled) 293T whole cell extract using LGR5 polyclonal antibody (Product # PA5-85589) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS PAGE.
Product Details
PA5-85589
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human LGR5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792729
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (84%), Rat (83%), Pig (89%), Bovine (86%).
Positive Control: Myc-human LGR5-transfected 293T (un-boiled).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
G protein-coupled receptor GPR49 has been reported to be expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries and from human germ cell, uterus, and brain libraries. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; Leucine-rich repeat-containing G-protein coupled receptor 5; orphan G protein-coupled receptor HG38
Gene Aliases: FEX; GPR49; GPR67; GRP49; HG38; LGR5
UniProt ID: (Human) O75473
Entrez Gene ID: (Human) 8549
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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