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LHX3 Antibody (MA5-37924) in WB
Western blot analysis of LHX3 in extracts of various cell lines. Samples were incubated with LHX3 Monoclonal antibody (Product # MA5-37924) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-37924
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2150
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human LHX3 (Q9UBR4)
Conjugate
Form
Liquid
Concentration
0.32 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897844
Product Specific Information
Positive Samples: HeLa, 293T, C6, SH-SY5Y, Mouse brain, Mouse kidney, Mouse uterus, Rat brain
Immunogen sequence: DSDAEVSFPD EPSLAEMGPA NGLYGSLGEP TQALGRPSGA LGNFSLEHGG LAGPEQYREL RPGSPYGVPP SPAAPQSLPG PQPLLSSLVY PDTSLGLVPS G
Target Information
This gene encodes a member a large protein family which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Mutations in this gene have been associated with a syndrome of combined pituitary hormone deficiency and rigid cervical spine. Two transcripts variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp762A2013; Homeobox protein LIM-3; Homeobox protein P-LIM; LIM homeo box protein 3; LIM homeobox protein 3; LIM/homeobox protein Lhx3; LIM/homeodomain protein LHX3
Gene Aliases: CPHD3; LHX3; Lim-3; LIM3; M2-LHX3; mLim-3; mLIM3; P-LIM; Plim
UniProt ID: (Human) Q9UBR4, (Mouse) P50481
Entrez Gene ID: (Human) 8022, (Rat) 170671, (Mouse) 16871
transcription factor activity, RNA polymerase II transcription factor binding
RNA polymerase II transcription factor binding
zinc ion binding
sequence-specific DNA binding
molecular_function
DNA binding
protein binding
metal ion binding
helix-turn-helix transcription factor
homeodomain transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
placenta development
transcription, DNA-templated
motor neuron axon guidance
organ morphogenesis
spinal cord motor neuron cell fate specification
ventral spinal cord interneuron specification
medial motor column neuron differentiation
spinal cord association neuron differentiation
pituitary gland development
lung development
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
dorsal/ventral pattern formation
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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