Invitrogen

LIF Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (19) LIF antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-79601

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF (175-194aa DKEAFQRKKLGCQLLGTYKQ).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746716

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cholinergic neuronal differentiation factor; D factor; Differentiation-stimulating factor; Leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; myeloid leukaemia inhibitory factor

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Gene Aliases: Lif

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UniProt ID: (Mouse) P09056

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Entrez Gene ID: (Mouse) 16878, (Rat) 60584

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Function(s)
transcription factor activity, RNA polymerase II transcription factor recruiting receptor binding cytokine activity leukemia inhibitory factor receptor binding growth factor activity cytokine intercellular signal molecule
Process(es)
blood vessel remodeling transcription from RNA polymerase II promoter immune response embryo implantation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of gene expression positive regulation of neuron projection development negative regulation of angiogenesis stem cell population maintenance lung development organ regeneration positive regulation of peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation of STAT protein tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of MAPK cascade regulation of cell differentiation positive regulation of macrophage differentiation negative regulation of meiotic nuclear division positive regulation of transcription from RNA polymerase II promoter decidualization negative regulation of hormone secretion lung alveolus development muscle organ morphogenesis neuron development astrocyte differentiation positive regulation of astrocyte differentiation leukemia inhibitory factor signaling pathway stem cell differentiation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of corticotropin secretion retina development in camera-type eye maternal process involved in female pregnancy transdifferentiation lung vasculature development lung lobe morphogenesis trophoblast giant cell differentiation spongiotrophoblast differentiation negative regulation of ERK1 and ERK2 cascade positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of protein localization to nucleus positive regulation of histone H3-K27 acetylation regulation of RNA polymerase II regulatory region sequence-specific DNA binding
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