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LIGHT Antibody (500-P179BT-25UG) in ELISA
Sandwich ELISA: To detect hLIGHT by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of LIGHT Polyclonal Antibody, Biotin (Product # 500-P179BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech LIGHT Polyclonal Antibody (Product # 500-P179-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hLIGHT.
Product Details
500-P179BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Insect cells-derived Recombinant Human LIGHT
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929670
Product Specific Information
AA Sequence of recombinant protein: RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIGHT. Anti-Human LIGHT-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hLIGHT by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human LIGHT (500-P179) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hLIGHT.
Western Blot: To detect Human LIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P179BT-1MG will be provided as 2 x 500 µg
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD258; Herpes virus entry mediator ligand; herpesvirus entry mediator ligand; HVEM-L; sCD258; sLIGHT; soluble CD258; soluble LIGHT; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; Tumor necrosis factor ligand superfamily member 14
Gene Aliases: CD258; HVEML; LIGHT; LTg; TNFSF14; UNQ391/PRO726
UniProt ID: (Human) O43557
Entrez Gene ID: (Human) 8740
apoptotic process
immune response
signal transduction
release of cytoplasmic sequestered NF-kappaB
positive regulation of T cell chemotaxis
T cell costimulation
tumor necrosis factor-mediated signaling pathway
T cell proliferation
T cell activation
T cell homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of myoblast differentiation
cellular response to mechanical stimulus
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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