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LIGHT Antibody (500-P308BT-25UG) in WB
Western Blot: To detect Murine LIGHT by Western Blot analysis LIGHT Polyclonal Antibody, Biotin (Product # 500-P308BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P308BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine LIGHT
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930324
Product Specific Information
AA Sequence of recombinant protein: MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA FMV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine LIGHT. Anti-Murine LIGHT-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine LIGHT by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine LIGHT (500-P308) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Murine LIGHT.
Western Blot: To detect Murine LIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P308BT-1MG will be provided as 2 x 500 µg
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD258; sCD258; sLIGHT; soluble CD258; soluble LIGHT; Tumor necrosis factor ligand superfamily member 14
Gene Aliases: HVEM-L; HVEML; LIGHT; LTg; Ly113; Tnfsf14
UniProt ID: (Mouse) Q9QYH9
Entrez Gene ID: (Mouse) 50930
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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