Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-85426
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human LSD1.
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792566
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (91%), Pig (99%), Rhesus Monkey (100%).
Positive Control: NT2D1, IMR32, MCF-7, PC-12, Rat2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; neuroprotective protein 3; RP1-184J9.1
Gene Aliases: AOF2; BHC110; CPRF; KDM1; KDM1A; KIAA0601; LSD1; RGD1562975
UniProt ID: (Human) O60341
Entrez Gene ID: (Human) 23028, (Rat) 500569
RNA polymerase II transcription factor binding
p53 binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
transcription factor binding
oxidoreductase activity
enzyme binding
ligand-dependent nuclear receptor transcription coactivator activity
demethylase activity
histone demethylase activity
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K9 specific)
histone demethylase activity (H3-dimethyl-K4 specific)
telomeric DNA binding
MRF binding
transcription regulatory region DNA binding
flavin adenine dinucleotide binding
androgen receptor binding
telomeric repeat-containing RNA binding
oxidase
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein demethylation
blood coagulation
cell proliferation
regulation of double-strand break repair via homologous recombination
regulation of primitive erythrocyte differentiation
histone deacetylation
pituitary gland development
granulocyte differentiation
negative regulation of protein binding
histone H3-K9 demethylation
positive regulation of histone ubiquitination
cellular response to UV
histone H3-K4 demethylation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of erythrocyte differentiation
positive regulation of megakaryocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
muscle cell development
oxidation-reduction process
cellular response to gamma radiation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of cellular protein localization
positive regulation of neural precursor cell proliferation
positive regulation of stem cell proliferation
alternative mRNA splicing, via spliceosome
positive regulation of neuron projection development
response to organic cyclic compound
cerebral cortex development
positive regulation of chromatin binding
neuron maturation
positive regulation of cell size
guanine metabolic process
response to fungicide
cellular response to cAMP
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support