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Product Details
39186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This antibody was raised against full-length recombinant human LSD1 / kDM1A protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2793175
Target Information
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; RP1-184J9.1
Gene Aliases: 1810043O07Rik; AA408884; AOF2; BHC110; CPRF; D4Ertd478e; KDM1; KDM1A; KIAA0601; LSD1; mKIAA0601
UniProt ID: (Human) O60341, (Mouse) Q6ZQ88
Entrez Gene ID: (Human) 23028, (Mouse) 99982
RNA polymerase II transcription factor binding
p53 binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
transcription factor binding
oxidoreductase activity
enzyme binding
ligand-dependent nuclear receptor transcription coactivator activity
demethylase activity
histone demethylase activity
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K9 specific)
histone demethylase activity (H3-dimethyl-K4 specific)
telomeric DNA binding
MRF binding
transcription regulatory region DNA binding
flavin adenine dinucleotide binding
androgen receptor binding
telomeric repeat-containing RNA binding
DNA binding
N-ethylmaleimide reductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
epoxyqueuosine reductase activity
oxidase
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein demethylation
blood coagulation
cell proliferation
regulation of double-strand break repair via homologous recombination
regulation of primitive erythrocyte differentiation
histone deacetylation
pituitary gland development
granulocyte differentiation
negative regulation of protein binding
histone H3-K9 demethylation
positive regulation of histone ubiquitination
cellular response to UV
histone H3-K4 demethylation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of erythrocyte differentiation
positive regulation of megakaryocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
muscle cell development
oxidation-reduction process
cellular response to gamma radiation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of cellular protein localization
positive regulation of neural precursor cell proliferation
positive regulation of stem cell proliferation
regulation of transcription, DNA-templated
multicellular organism development
positive regulation of neuron projection development
chromatin modification
positive regulation of chromatin binding
positive regulation of cell size
guanine metabolic process
negative regulation of transcription from RNA polymerase II promoter by histone modification
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