Invitrogen

Lamin A/C Recombinant Rabbit Monoclonal Antibody (4L8Q0)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (51) Lamin A/C antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Lamin A/C Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Lamin A/C Antibody (MA5-35284) in ICC/IF

Confocal immunofluorescence (Immunocytochemistry) analysis of Lamin A/C in HeLa cells. Samples were incubated with Lamin A/C Monoclonal antibody (Product # MA5-35284) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Lamin A/C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Lamin A/C Antibody in Immunoprecipitation (IP)

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Product Details

MA5-35284

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50,000-1:300,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:4,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

0.5-4 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4L8Q0

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_7338211)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2849186

Product Specific Information

Immunogen sequence: SSHSSQTQGG GSVTKKRKLE STESRSSFSQ HARTSGRVAV EEVDEEGKFV RLRNKSNEDQ SMGNWQIKRQ NGDDPLLTYR FPPKFTLKAG QVVTIWAAGA GATHSPPTDL VWKAQNTWGC GNSLRTALIN STGEEVAMRK LVRSVTVVED DEDEDGDDLL HHHHGSHCSS

Target Information

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleoporin downregulation modulates progenitor differentiation independent of nuclear pore numbers.

Communications biology

Neely AE,Zhang Y,Blumensaadt LA,Mao H,Brenner B,Sun C,Zhang HF,Bao X

MA5-35284 was used in Western Blot to highlight the gene regulatory roles contributed by differential NUP expression levels in keratinocyte differentiation, independent of nuclear pore numbers.

Wed Oct 18 00:00:00 EDT 2023

Transcriptome Analysis Reveals Vimentin-Induced Disruption of Cell-Cell Associations Augments Breast Cancer Cell Migration.

Cells

Usman S,Jamal A,Bushaala A,Waseem NH,Al-Dehlawi H,Yeudall WA,Teh MT,Tummala H,Waseem A

MA5-35284 was used in Western Blotting to identify a vimentin-specific signature consisting of 13 protein encoding genes (CDH5, AXL, PTPRM, TGFBI, CDH10, NES, E2F1, FOXM1, CDC45, FSD1, BCL2, KIF26A and WISP2) and two long non-coding RNAs, LINC00052 and C15ORF9-AS1.

Tue Dec 13 00:00:00 EST 2022

Bioinformatics

Protein Aliases: 70 kDa lamin; lamin A; Lamin A+C mutant; lamin a-c; lamin A/C-like 1; Lamin AC; lamin C; lamin C2; lamin-A/C; mutant 453W; Mutant lamin A/C; Prelamin-A/C; progerin mutant; renal carcinoma antigen NY-REN-32; RP11-54H19.1

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Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1

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UniProt ID: (Human) P02545, (Mouse) P48678

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Entrez Gene ID: (Human) 4000, (Mouse) 16905, (Rat) 60374

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Function(s)

structural molecule activity protein binding protein phosphatase 1 binding

Process(es)

nucleus organization nuclear envelope organization muscle organ development establishment of cell polarity regulation of cell migration establishment or maintenance of microtubule cytoskeleton polarity protein localization to nucleus sterol regulatory element binding protein import into nucleus ventricular cardiac muscle cell development negative regulation of release of cytochrome c from mitochondria positive regulation of cell aging regulation of protein localization to nucleus negative regulation of extrinsic apoptotic signaling pathway mitotic nuclear envelope disassembly mitotic nuclear envelope reassembly spermatogenesis response to mechanical stimulus IRE1-mediated unfolded protein response positive regulation of osteoblast differentiation cellular response to hypoxia negative regulation of adipose tissue development

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Lamin A/C Recombinant Rabbit Monoclonal Antibody (4L8Q0)

Lamin A/C Antibody (MA5-35284) in ICC/IF

Product Details

MA5-35284

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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