Invitrogen

Lamin A/C Monoclonal Antibody (mab636)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

16 Published Figures

31 References

View all (51) Lamin A/C antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Lamin A/C Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

Lamin A/C Antibody (MA3-1000) in ICC/IF

Immunofluorescent analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product # MA3-1000), at a dilution of 1:100 for at leas... View More

Lamin A/C Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Lamin A/C Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Lamin A/C Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Lamin A/C Antibody in Immunohistochemistry (IHC)

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Product Details

MA3-1000

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:100

View 10 publications 10 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

mab636

Immunogen

Porcine lamin preparation.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325377

Product Specific Information

MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples.

MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.

The MA3-1000 antigen is porcine lamin preparation.

Target Information

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis.

NPJ Regenerative medicine

Stearns-Reider KM,Hicks MR,Hammond KG,Reynolds JC,Maity A,Kurmangaliyev YZ,Chin J,Stieg AZ,Geisse NA,Hohlbauch S,Kaemmer S,Schmitt LR,Pham TT,Yamauchi K,Novitch BG,Wollman R,Hansen KC,Pyle AD,Crosbie RH

MA3-1000 was used in Immunohistochemistry to investigate whether fibrotic ECM scarring affected human skeletal muscle progenitor cell (SMPC) functions that are essential for myoregeneration.

Wed Mar 15 00:00:00 EDT 2023

RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines.

Journal of bone oncology

Betlej G,Ząbek T,Lewińska A,Błoniarz D,Rzeszutek I,Wnuk M

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunocytochemistry

Sat Oct 01 00:00:00 EDT 2022

RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines.

Journal of bone oncology

Betlej G,Ząbek T,Lewińska A,Błoniarz D,Rzeszutek I,Wnuk M

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunocytochemistry

Sat Oct 01 00:00:00 EDT 2022

Human pluripotent stem cell-derived myogenic progenitors undergo maturation to quiescent satellite cells upon engraftment.

Cell stem cell

Sun C,Kannan S,Choi IY,Lim H,Zhang H,Chen GS,Zhang N,Park SH,Serra C,Iyer SR,Lloyd TE,Kwon C,Lovering RM,Lim SB,Andersen P,Wagner KR,Lee G

MA3-1000 was used in Immunohistochemistry to demonstrate that a subset of PAX7::GFP MPCs engraft within the basal lamina of regenerated myofibers, adopt a quiescent state, and contribute to regeneration upon reinjury and in mdx mouse models.

Thu Apr 07 00:00:00 EDT 2022

iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling.

eLife

Guo D,Daman K,Chen JJ,Shi MJ,Yan J,Matijasevic Z,Rickard AM,Bennett MH,Kiselyov A,Zhou H,Bang AG,Wagner KR,Maehr R,King OD,Hayward LJ,Emerson CP

MA3-1000 was used in Immunocytochemistry-immunoflourescence to establish the utility of iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease pathogenesis and for the development of muscle stem cell therapeutics.

Tue Jan 25 00:00:00 EST 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

MA3-1000 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to conclude that CircATXN7 accelerated the malignancy of NSCLC cells through adsorbing miR-7-5p and upregulating PFN2, offering evidence to support circATXN7 as a target for NSCLC treatment.

Wed Jun 01 00:00:00 EDT 2022

HMGB1 enhances chemotherapy resistance in multiple myeloma cells by activating the nuclear factor-κB pathway.

Experimental and therapeutic medicine

Ning J,Yang R,Wang H,Cui L

MA3-1000 was used in Western Blotting to explore molecular mechanism underlying HMGB1-mediated drug resistance in multiple myeloma (MM).

Thu Jul 01 00:00:00 EDT 2021

HMGB1 enhances chemotherapy resistance in multiple myeloma cells by activating the nuclear factor-κB pathway.

Experimental and therapeutic medicine

Ning J,Yang R,Wang H,Cui L

MA3-1000 was used in Western Blotting to explore molecular mechanism underlying HMGB1-mediated drug resistance in multiple myeloma (MM).

Thu Jul 01 00:00:00 EDT 2021

A human infertility-associated KASH5 variant promotes mitochondrial localization.

Scientific reports

Bentebbal SA,Meqbel BR,Salter A,Allan V,Burke B,Horn HF

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunocytochemistry

Wed May 12 00:00:00 EDT 2021

Surface Roughness Gradients Reveal Topography-Specific Mechanosensitive Responses in Human Mesenchymal Stem Cells.

Small (Weinheim an der Bergstrasse, Germany)

Hou Y,Xie W,Yu L,Camacho LC,Nie C,Zhang M,Haag R,Wei Q

MA3-1000 was used in Immunocytochemistry to study the mechanotransduction and fate determination of human mesenchymal stem cells (MSCs) on surface roughness gradients.

Sun Mar 01 00:00:00 EST 2020

Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine.

Frontiers in cell and developmental biology

Yang F,Liu Q,Chen Y,Ye H,Wang H,Zeng S

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunohistochemistry

Tue Feb 02 00:00:00 EST 2021

Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine.

Frontiers in cell and developmental biology

Yang F,Liu Q,Chen Y,Ye H,Wang H,Zeng S

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunohistochemistry

Tue Feb 02 00:00:00 EST 2021

Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine.

Frontiers in cell and developmental biology

Yang F,Liu Q,Chen Y,Ye H,Wang H,Zeng S

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Immunohistochemistry

Tue Feb 02 00:00:00 EST 2021

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

MA3-1000 was used in Immunohistochemistry to demonstrate that endogenous activation of the PAX7 transcription factor results in stable epigenetic remodeling and differentiates human pluripotent stem cells into skeletal myogenic progenitor cells.

Tue May 12 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Tue May 12 00:00:00 EDT 2020

Human MuStem Cell Grafting into Infarcted Rat Heart Attenuates Adverse Tissue Remodeling and Preserves Cardiac Function.

Molecular therapy. Methods & clinical development

Rannou A,Toumaniantz G,Larcher T,Leroux I,Ledevin M,Hivonnait A,Babarit C,Fleurisson R,Dubreil L,Ménoret S,Anegon I,Charpentier F,Rouger K,Guével L

MA3-1000 was used in Immunohistochemistry to study the transplantation of hMuStem cells in an immunodeficient rat model of myocardial infarction by providing an extensive histological and molecular analysis of their behavior 3 weeks after local administration.

Fri Sep 11 00:00:00 EDT 2020

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7.

International journal of molecular sciences

Alghanem B,Ali R,Nehdi A,Al Zahrani H,Altolayyan A,Shaibah H,Baz O,Alhallaj A,Moresco JJ,Diedrich JK,Yates JR,Boudjelal M

MA3-1000 was used in Western Blotting to perform a Tandem Mass Tag (TMT) strategy for accurate peptide/protein quantification.

Thu Jun 18 00:00:00 EDT 2020

Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7.

International journal of molecular sciences

Alghanem B,Ali R,Nehdi A,Al Zahrani H,Altolayyan A,Shaibah H,Baz O,Alhallaj A,Moresco JJ,Diedrich JK,Yates JR,Boudjelal M

MA3-1000 was used in Western Blotting to perform a Tandem Mass Tag (TMT) strategy for accurate peptide/protein quantification.

Thu Jun 18 00:00:00 EDT 2020

Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia.

Disease markers

Sakowicz A,Lisowska M,Biesiada L,Płuciennik E,Gach A,Rybak-Krzyszkowska M,Huras H,Sakowicz B,Romanowicz H,Piastowska-Ciesielska AW,Grzesiak M,Pietrucha T

MA3-1000 was used in Western Blotting to examine the level and localisation of the NEMO protein in preeclamptic and nonpreeclamptic placentas.

Wed May 29 00:00:00 EDT 2019

A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration.

The Journal of biological chemistry

Vo TM,Jain S,Burchett R,Monckton EA,Godbout R

MA3-1000 was used in Western Blotting to identify the existence of a positive feedback loop between nuclear factor IB and calpain 1 in glioblastoma cells which suppresses cell migration: a potential therapeutic target for controlling of tumor mass, but not migration or infiltration.

Fri Aug 23 00:00:00 EDT 2019

A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration.

The Journal of biological chemistry

Vo TM,Jain S,Burchett R,Monckton EA,Godbout R

Fri Aug 23 00:00:00 EDT 2019

Inhibition of JAK-STAT Signaling with Baricitinib Reduces Inflammation and Improves Cellular Homeostasis in Progeria Cells.

Cells

Liu C,Arnold R,Henriques G,Djabali K

MA3-1000 was used in Immunocytochemistry to report that the inhibition of the JAK-STAT pathway with baricitinib, a Food and Drug Administration-approved JAK1/2 inhibitor, restored cellular homeostasis, delayed senescence and decreased proinflammatory markers in HGPS cells.

Fri Oct 18 00:00:00 EDT 2019

TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells.

Frontiers in immunology

Joshi RN,Stadler C,Lehmann R,Lehtiö J,Tegnér J,Schmidt A,Vesterlund M

MA3-1000 was used in Western Blotting sorting to provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

Mon Nov 09 00:00:00 EST 2020

Mechanical stability of the cell nucleus - roles played by the cytoskeleton in nuclear deformation and strain recovery.

Journal of cell science

Wang X,Liu H,Zhu M,Cao C,Xu Z,Tsatskis Y,Lau K,Kuok C,Filleter T,McNeill H,Simmons CA,Hopyan S,Sun Y

MA3-1000 was used in Immunocytochemistry-immunoflourescence to understand that cytoskeleton enhances nuclear mechanical stability by lowering the effective deformability of the nucleus but keeping nuclear sensitivity to mechanical stimuli.

Wed Jul 04 00:00:00 EDT 2018

Upregulation of tryptophanyl-tRNA synthethase adapts human cancer cells to nutritional stress caused by tryptophan degradation.

Oncoimmunology

Adam I,Dewi DL,Mooiweer J,Sadik A,Mohapatra SR,Berdel B,Keil M,Sonner JK,Thedieck K,Rose AJ,Platten M,Heiland I,Trump S,Opitz CA

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Western Blot

Tue Jan 12 00:00:00 EST 2021

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Cancer & metabolism

Koufaris C,Nilsson R

Published figure using Lamin A/C monoclonal antibody (Product # MA3-1000) in Western Blot

Mon Mar 21 00:00:00 EDT 2022

Exploring Modifications of an HIV-1 Capsid Inhibitor: Design, Synthesis, and Mechanism of Action.

Journal of drug design and research

Xu JP,Francis AC,Meuser ME,Mankowski M,Ptak RG,Rashad AA,Melikyan GB,Cocklin S

MA3-1000 was used in Immunocytochemistry-immunoflourescence to investigate bioisosteric modification of the the HIV-1 caspid inhibitor PF-3450074 scaffold as a first step in redeveloping this compound.

Thu Oct 01 00:00:00 EDT 2020

Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma.

Glia

Liu RZ,Li S,Garcia E,Glubrecht DD,Poon HY,Easaw JC,Godbout R

MA3-1000 was used in western blot to determine poor prognosis markers in glioblastoma involving altered retinoic acid signaling and an association with cytoplasmic CRABP2

Wed Jun 01 00:00:00 EDT 2016

Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.

Journal of virology

DeRussy BM,Boland MT,Tandon R

MA3-1000 was used in western blot to determine the link between nuclear egress and nucleocapsid maturation by human cytomegalovirus pUL93

Mon Aug 15 00:00:00 EDT 2016

Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts.

Oncotarget

Eisch V,Lu X,Gabriel D,Djabali K

MA3-1000 was used in western blot test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts

Tue Apr 26 00:00:00 EDT 2016

Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity.

eLife

Heo SJ,Driscoll TP,Thorpe SD,Nerurkar NL,Baker BM,Yang MT,Chen CS,Lee DA,Mauck RL

MA31000 was used in immunocytochemistry and western blot to investigate the mechanosensitivity of mesenchymal stem cell

Wed Nov 30 00:00:00 EST 2016

Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity.

eLife

Heo SJ,Driscoll TP,Thorpe SD,Nerurkar NL,Baker BM,Yang MT,Chen CS,Lee DA,Mauck RL

MA31000 was used in immunocytochemistry and western blot to investigate the mechanosensitivity of mesenchymal stem cell

Wed Nov 30 00:00:00 EST 2016

Perinuclear localization of the HIV-1 regulatory protein Vpr is important for induction of G2-arrest.

Virology

Sörgel S,Fraedrich K,Votteler J,Thomas M,Stamminger T,Schubert U

MA3-1000 was used in immunocytochemistry to study the role of the HIV regulatory protein Vpr in G2 arrest

Thu Oct 25 00:00:00 EDT 2012

Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12.

Journal of cellular biochemistry

Lee JH,Zhou S,Smas CM

MA3-1000 was used in western blot to investigate the effect of RANBP 16 and 17 on bHLH transcription factor E12 function

Wed Sep 01 00:00:00 EDT 2010

Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex.

Cell motility and the cytoskeleton

Lindsay AJ,McCaffrey MW

MA3-1000 was used in western blot to study the localization and functions of myosin Va and Vb

Tue Dec 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 70 kDa lamin; Lamin A+C mutant; lamin a-c; lamin A/C-like 1; Lamin AC; mutant 453W; Mutant lamin A/C; Prelamin-A/C; progerin mutant; renal carcinoma antigen NY-REN-32; RP11-54H19.1; unnamed protein product

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Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1

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UniProt ID: (Human) P02545

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Entrez Gene ID: (Bovine) 404144, (Pig) 100126859, (Human) 4000

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Function(s)

structural molecule activity protein binding protein phosphatase 1 binding

Process(es)

mitotic nuclear envelope disassembly mitotic nuclear envelope reassembly spermatogenesis response to mechanical stimulus regulation of cell migration establishment or maintenance of microtubule cytoskeleton polarity protein localization to nucleus sterol regulatory element binding protein import into nucleus IRE1-mediated unfolded protein response positive regulation of osteoblast differentiation ventricular cardiac muscle cell development cellular response to hypoxia negative regulation of release of cytochrome c from mitochondria positive regulation of cell aging regulation of protein localization to nucleus negative regulation of adipose tissue development negative regulation of extrinsic apoptotic signaling pathway

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Lamin A/C Monoclonal Antibody (mab636)

Lamin A/C Antibody (MA3-1000) in ICC/IF

Product Details

MA3-1000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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