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Invitrogen
Lamin B1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Lamin B1 Antibody (PA5-95850) in WB
Western blot analysis of Lamin B1 in extracts of various cell lines. Samples were incubated with Lamin B1 Polyclonal antibody (Product # PA5-95850) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-95850
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 503-586 of human Lamin B1 (NP_0055641)
Conjugate
Form
Liquid
Concentration
3.03 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807652
Product Specific Information
Positive Samples: Jurkat, HT-29, HeLa, U-251MG, mouse liver, mouse brain, mouse spleen, rat liver
Immunogen sequence: AGVTASPPTD LIWKNQNSWG TGEDVKVILK NSQGEEVAQR STVFKTTIPE EEEEEEEAAG VVVEEELFHQ QGTPRASNRS CAIM
Target Information
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lamin-B1; MGC111419
Gene Aliases: ADLD; LMN; LMN2; LMNB; LMNB1
UniProt ID: (Human) P20700, (Mouse) P14733, (Rat) P70615
Entrez Gene ID: (Human) 4001, (Mouse) 16906, (Rat) 116685
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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