Invitrogen

Lamin B1 Monoclonal Antibody (119D5-F1)

Lamin B1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 12

Lamin B1 Antibody (MA1-06103) in IHC (F)

Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells stained with Lamin B1 monoclonal antibody (Product # MA1-06103).

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Product Details

MA1-06103

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

Immunocytochemistry (ICC/IF)

1:100-1:200

Flow Cytometry (Flow)

1:100-1:200

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat, Zebrafish

Published species

Bovine, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

119D5-F1

Immunogen

Purified rat liver lamin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2281281

Product Specific Information

MA1-06103 detects lamin B in human, mouse, rat, bovine, ovine, rabbit, zebrafish, and canine samples.

MA1-06103 has sucessfully been used in immunocytochemistry, immunohistochemistry, ELISA, and flow cytometry procedures.

The MA1-06103 immunogen is purified rat liver lamins.

Target Information

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Defects in the LMNB1 gene can cause Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson's Disease Models.

Cells

Verma DK,Seo BA,Ghosh A,Ma SX,Hernandez-Quijada K,Andersen JK,Ko HS,Kim YH

MA1-06103 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to indicate that PFF-induced pathology could lead to astrocyte and/or microglia senescence in PD brains, which may contribute to neuropathology in this model.

Mon Jul 05 00:00:00 EDT 2021

Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson's Disease Models.

Cells

Verma DK,Seo BA,Ghosh A,Ma SX,Hernandez-Quijada K,Andersen JK,Ko HS,Kim YH

Mon Jul 05 00:00:00 EDT 2021

MacroH2A histone variants maintain nuclear organization and heterochromatin architecture.

Journal of cell science

Douet J,Corujo D,Malinverni R,Renauld J,Sansoni V,Posavec Marjanović M,Cantariño N,Valero V,Mongelard F,Bouvet P,Imhof A,Thiry M,Buschbeck M

MA106103 was used in ChIP assay, proximity ligation assay, and western blot to show that macroH2A variants aid in maintaining nuclear organization and heterochromatin architecture

Mon May 01 00:00:00 EDT 2017

Immunoregulatory Protein B7-H3 Reprograms Glucose Metabolism in Cancer Cells by ROS-Mediated Stabilization of HIF1α.

Cancer research

Lim S,Liu H,Madeira da Silva L,Arora R,Liu Z,Phillips JB,Schmitt DC,Vu T,McClellan S,Lin Y,Lin W,Piazza GA,Fodstad O,Tan M

MA1-06103 was used in western blot to study the reprogramming of glucose metabolism in cancer cells by ROS-mediated stabilization of HIF1alpha via the immunoregulatory protein B7-H3

Fri Apr 15 00:00:00 EDT 2016

Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.

PloS one

Janjanam J,Singh S,Jena MK,Varshney N,Kola S,Kumar S,Kaushik JK,Grover S,Dang AK,Mukesh M,Prakash BS,Mohanty AK

MA1-06103 was used in western blot to use 2D-DIGE and mass spectrometry to identify the protein changes in mammary epithelial cells

Wed Apr 15 00:00:00 EDT 2015

Receptor tyrosine kinase ErbB2 translocates into mitochondria and regulates cellular metabolism.

Nature communications

Ding Y,Liu Z,Desai S,Zhao Y,Liu H,Pannell LK,Yi H,Wright ER,Owen LB,Dean-Colomb W,Fodstad O,Lu J,LeDoux SP,Wilson GL,Tan M

MA1-06103 was used in western blot to study the function of mitochondrial ErbB2

Tue Jun 11 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Lamin-B1; MGC111419

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Gene Aliases: ADLD; fc06g01; LMN; LMN2; LMNB; LMNB1; wu:fc06g01

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UniProt ID: (Human) P20700, (Mouse) P14733, (Rat) P70615

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Entrez Gene ID: (Rabbit) 100346094, (Human) 4001, (Sheep) 100037680, (Dog) 474663, (Bovine) 540643, (Mouse) 16906, (Rat) 116685, (Zebrafish) 195816

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Function(s)

structural molecule activity phospholipase binding JUN kinase binding

Process(es)

positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle positive regulation of JNK cascade

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Lamin B1 Monoclonal Antibody (119D5-F1)

Lamin B1 Antibody (MA1-06103) in IHC (F)

Product Details

MA1-06103

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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