Invitrogen

Leptin Polyclonal Antibody, PeproTech®

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View all (34) Leptin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

500-P86-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
View 3 publications 3 publications

Radioimmune Assays (RIA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Leptin
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930816

Product Specific Information

AA Sequence of recombinant protein: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Leptin. Anti-Human Leptin-specific antibody was purified by affinity chromatography employing an immobilized Human Leptin matrix.

Sandwich ELISA: To detect Human Leptin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Leptin (500-P86Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Leptin.

Western Blot: To detect Human Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Leptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD295 antigen; H-Leptin; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein

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Gene Aliases: LEP; LEPD; OB; OBS

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UniProt ID: (Human) P41159

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Entrez Gene ID: (Human) 3952

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Function(s)
hormone activity growth factor activity peptide hormone receptor binding peptide hormone
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis ovulation from ovarian follicle response to hypoxia positive regulation of cytokine production placenta development regulation of endothelial cell proliferation response to dietary excess cardiac muscle hypertrophy glucose metabolic process regulation of gluconeogenesis energy reserve metabolic process glycerol biosynthetic process lipid metabolic process fatty acid beta-oxidation tyrosine phosphorylation of STAT protein female pregnancy circadian rhythm cholesterol metabolic process bile acid metabolic process regulation of blood pressure adult feeding behavior negative regulation of autophagy negative regulation of lipid storage positive regulation of phosphatidylinositol 3-kinase signaling response to activity sexual reproduction central nervous system neuron development insulin secretion T cell differentiation regulation of intestinal cholesterol absorption positive regulation of TOR signaling negative regulation of appetite prostaglandin secretion response to estradiol regulation of natural killer cell activation regulation of natural killer cell proliferation response to insulin response to vitamin E leptin-mediated signaling pathway positive regulation of luteinizing hormone secretion positive regulation of peroxisome proliferator activated receptor signaling pathway bone mineralization involved in bone maturation negative regulation of appetite by leptin-mediated signaling pathway positive regulation of T cell proliferation regulation of natural killer cell mediated cytotoxicity hormone metabolic process positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis eating behavior negative regulation of apoptotic process positive regulation of ion transport positive regulation of MAPK cascade cellular response to leptin stimulus response to ethanol positive regulation of myeloid cell differentiation regulation of angiogenesis negative regulation of vasoconstriction negative regulation of glucose import positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway regulation of bone remodeling positive regulation of follicle-stimulating hormone secretion positive regulation of developmental growth regulation of insulin secretion regulation of steroid biosynthetic process intestinal absorption leukocyte tethering or rolling regulation of nitric-oxide synthase activity regulation of cell cycle positive regulation of protein kinase B signaling regulation of lipoprotein lipid oxidation adipose tissue development negative regulation of cartilage development negative regulation of glucagon secretion cellular response to L-ascorbic acid cellular response to retinoic acid interleukin-6 secretion interleukin-8 secretion regulation of brown fat cell differentiation bone growth regulation of cytokine production involved in inflammatory response positive regulation of p38MAPK cascade positive regulation of fat cell apoptotic process activation of protein kinase C activity positive regulation of STAT protein import into nucleus positive regulation of reactive oxygen species metabolic process negative regulation of glutamine transport positive regulation of hepatic stellate cell activation
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