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Product Details
MA5-49058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F1
Immunogen
The monoclonal antibody 2F1 reacts with human soluble leptin receptor (sLR) in plasma
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_3091120
Product Specific Information
The monoclonal antibody 2F1 specifically reacts with sLR with a molecular mass of 180kD. The 2F1 antibody can be used to measure in both free sLR and sLR bound to leptin in plasma.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen CD295; CD295; CD295 antigen; HuB219; HuB219.2; LEP-R; Leptin receptor; OB receptor; OB-R
Gene Aliases: CD295; DB; LEP-R; LEPR; LEPRD; OB-R; OBR
UniProt ID: (Human) P48357
Entrez Gene ID: (Human) 3953
angiogenesis
energy reserve metabolic process
cell surface receptor signaling pathway
multicellular organism development
cholesterol metabolic process
negative regulation of autophagy
sexual reproduction
T cell differentiation
leptin-mediated signaling pathway
glucose homeostasis
response to leptin
negative regulation of gluconeogenesis
regulation of bone remodeling
negative regulation of hydrolase activity
regulation of feeding behavior
energy homeostasis
bone growth
regulation of energy homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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