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Invitrogen
Ly-49E/F Monoclonal Antibody (CM4), APC, eBioscience™
FIGURE: 1 / 2
Ly-49E/F Antibody (17-5848-80) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse NK1.1 PE (Product # 12-5941-82) and 0.125 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse Ly-49E/F APC (right). Total viable cells were used for analysis.
Product Details
17-5848-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CM4
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10557243
Product Specific Information
Description: This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F. The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).
Applications Reported: This CM4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CM4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class I MHC receptor KLRA5; Killer cell lectin-like receptor 5; Killer cell lectin-like receptor 6; Ly-49e; Ly-49f; Ly49E receptor; Lymphocyte antigen 49e; Lymphocyte antigen 49f; T-cell surface glycoprotein Ly-49E; T-cell surface glycoprotein Ly-49F
Gene Aliases: Klra5; Klra6; Ly-49e; Ly-49f; Ly49-e; Ly49-f; Ly49e; Ly49F
UniProt ID: (Mouse) Q60652, (Mouse) Q60653
Entrez Gene ID: (Mouse) 16636, (Mouse) 16637
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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