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Invitrogen
Ly-49G Monoclonal Antibody (AT-8 (AT8)), PE, eBioscience™
FIGURE: 1 / 1
Ly-49G Antibody (12-5885-82) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.25 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or 0.5 µg of Anti-Mouse Ly-49G PE (right). Total viable cells were used for analysis.
Product Details
12-5885-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AT-8 (AT8)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466005
Product Specific Information
Description: The AT8 monoclonal antibody reacts with the mouse Ly-49G receptor from 129, FVB, BALB/c, C3H, CBA, SJL and A/J, but not C57Bl/6 mice. Ly49G, a member of the Ly49 family is expressed by NK cell subset.
Applications Reported: The AT-8 (AT8) antibody has been reported for use in flow cytometric analysis.
Applications Tested: The AT-8 (AT8) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Killer cell lectin-like receptor 7; Ly-49g; Lymphocyte antigen 49g; T-cell surface glycoprotein Ly-49G
Gene Aliases: Klra7; LGL-1; Ly-49g; Ly-49G.1; Ly-49G.2; Ly-49G.3; Ly49-g; Ly49g; Ly49G.4; Ly49g1; Ly49g2; Ly49g4
UniProt ID: (Mouse) Q60654
Entrez Gene ID: (Mouse) 16638
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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