Invitrogen

Ly-49H Monoclonal Antibody (3D10), eBioscience™

Ly-49H Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

Ly-49H Antibody (14-5886-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971) and 0.125 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714) (left) or 0.125 µg of Anti-Mouse Ly-49H Purified (right) followed by Anti-Mouse IgG Biotin and Streptavidin APC (Product # 17-4317).Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

14-5886-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 11 publications 11 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_906245

Product Specific Information

Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D(Product # 12-5783-81) . Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.

Applications Reported:This 3D10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D10 antibody has been tested by flow cytometric analyis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using Ly-49H monoclonal antibody (Product # 14-5886-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

14-5886 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 EDT 2020

Natural killer cells attenuate cytomegalovirus-induced hearing loss in mice.

PLoS pathogens

Almishaal AA,Mathur PD,Hillas E,Chen L,Zhang A,Yang J,Wang Y,Yokoyama WM,Firpo MA,Park AH

Published figure using Ly-49H monoclonal antibody (Product # 14-5886-82) in Neutralization

Tue Aug 01 00:00:00 EDT 2017

Natural killer cells attenuate cytomegalovirus-induced hearing loss in mice.

PLoS pathogens

Almishaal AA,Mathur PD,Hillas E,Chen L,Zhang A,Yang J,Wang Y,Yokoyama WM,Firpo MA,Park AH

Published figure using Ly-49H monoclonal antibody (Product # 14-5886-82) in Neutralization

Tue Aug 01 00:00:00 EDT 2017

TAP-independent self-peptides enhance T cell recognition of immune-escaped tumors.

The Journal of clinical investigation

Doorduijn EM,Sluijter M,Querido BJ,Oliveira CC,Achour A,Ossendorp F,van der Burg SH,van Hall T

14-5886 was used in Flow cytometry/Cell sorting to analyse thymus selection and peripheral behaviour of T cells with specificity for the self-antigen known as T cell epitopes associated with impaired peptide processing (TEIPP).

Mon Feb 01 00:00:00 EST 2016

NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3.

Journal of immunology (Baltimore, Md. : 1950)

Hamblet CE,Makowski SL,Tritapoe JM,Pomerantz JL

14-5886 was used in Flow cytometry/Cell sorting to investigate the role of SPPL3 in murine NK cell biology.

Tue Mar 15 00:00:00 EDT 2016

BNIP3- and BNIP3L-Mediated Mitophagy Promotes the Generation of Natural Killer Cell Memory.

Immunity

O'Sullivan TE,Johnson LR,Kang HH,Sun JC

14-5886 was used in Flow cytometry/Cell sorting to reveal the functional importance of mitophagy during the dynamic response of these cytolytic innate lymphocytes.

Tue Aug 18 00:00:00 EDT 2015

Cytokine-Mediated Activation of NK Cells during Viral Infection.

Journal of virology

Freeman BE,Raué HP,Hill AB,Slifka MK

14-5886 was used in Flow cytometry/Cell sorting to study how cytokines interact to regulate natural killer cell function during viral infection.

Sat Aug 01 00:00:00 EDT 2015

The Critical Role of IL-15-PI3K-mTOR Pathway in Natural Killer Cell Effector Functions.

Frontiers in immunology

Nandagopal N,Ali AK,Komal AK,Lee SH

14-5886 was used in Functional assays to investigate whether the effect of IL-15 priming of NK cells can be extended to other cytokines, showing a critical role of the IL-15-PI3K-mTOR in NK effector functions.

Mon May 05 00:00:00 EDT 2014

Tissue-resident natural killer (NK) cells are cell lineages distinct from thymic and conventional splenic NK cells.

eLife

Sojka DK,Plougastel-Douglas B,Yang L,Pak-Wittel MA,Artyomov MN,Ivanova Y,Zhong C,Chase JM,Rothman PB,Yu J,Riley JK,Zhu J,Tian Z,Yokoyama WM

14-5886 was used in Flow cytometry/Cell sorting to investigate whether tissue-resident and cells resembling conventional splenic natural killer cells natural killer cells are derived from the same lineage, showing that there are at least four distinct lineages.

Wed Jan 01 00:00:00 EST 2014

Molecular definition of the identity and activation of natural killer cells.

Nature immunology

Bezman NA,Kim CC,Sun JC,Min-Oo G,Hendricks DW,Kamimura Y,Best JA,Goldrath AW,Lanier LL

14-5886 was used in Flow cytometry/Cell sorting to systematically define the transcriptome of mouse natural killer cells in several contexts.

Mon Oct 01 00:00:00 EDT 2012

Protein kinase C-θ clustering at immunological synapses amplifies effector responses in NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Merino E,Abeyweera TP,Firth MA,Zawislak CL,Basu R,Liu X,Sun JC,Huse M

14-5886 was used in Functional assays to study the role of compartmentalised PKC signalling in natural killer cell activation.

Thu Nov 15 00:00:00 EST 2012

A novel Ncr1-Cre mouse reveals the essential role of STAT5 for NK-cell survival and development.

Blood

Eckelhart E,Warsch W,Zebedin E,Simma O,Stoiber D,Kolbe T,Rülicke T,Mueller M,Casanova E,Sexl V

14-5886 was used in Flow cytometry/Cell sorting to develop a novel mouse line to delineate the essential and cell-intrinsic role of STAT5 in NK-cell development.

Thu Feb 03 00:00:00 EST 2011

Genetic labeling reveals altered turnover and stability of innate lymphocytes in latent mouse cytomegalovirus infection.

Journal of immunology (Baltimore, Md. : 1950)

Busche A,Schmitz S,Fleige H,Robbins SH,Walzer T,Stewart CA,Förster R,Messerle M,Prinz I

14-5886 was used in Flow cytometry/Cell sorting to determine that antiviral immune responses may induce sustained alterations in natural killer cell populations.

Tue Mar 01 00:00:00 EST 2011

Ly49H signaling through DAP10 is essential for optimal natural killer cell responses to mouse cytomegalovirus infection.

The Journal of experimental medicine

Orr MT,Sun JC,Hesslein DG,Arase H,Phillips JH,Takai T,Lanier LL

14-5886 was used in Flow cytometry/Cell sorting to study Ly49H signaling through DAP10 for optimal natural killer cell responses to mouse cytomegalovirus infection.

Mon Apr 13 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: cytomegalovirus resistance 1; Killer cell lectin-like receptor 8; Ly-49h; Lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H

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Gene Aliases: Cmv-1; Cmv1; Klra8; Ly-49h; Ly49-h; Ly49H; Ly49u<129>

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UniProt ID: (Mouse) Q60682

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Entrez Gene ID: (Mouse) 16639

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Function(s)

carbohydrate binding

Process(es)

cell adhesion response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ly-49H Monoclonal Antibody (3D10), eBioscience™

Ly-49H Antibody (14-5886-82) in Flow

Product Details

14-5886-82

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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