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Invitrogen
Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™
FIGURE: 1 / 2
Ly-49I Antibody (11-5895-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or 0.5 µg of Anti-Mouse Ly-49I FITC (right). Samples were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82). Total viable cells were used for analysis.
Product Details
11-5895-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
YLI-90
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465301
Product Specific Information
Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice.
Applications Reported: YLI-90 has been reported for use in flow cytometric analysis.
Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: 5E6; Killer cell lectin-like receptor 3; killer cell lectin-like receptor subfamily A, member 9; Ly-49; Ly-49c; Ly-49I receptor; Ly49I receptor; Lymphocyte antigen 49c; Nk2.1; T-cell surface glycoprotein Ly-49C
Gene Aliases: Klra3; Ly-49c; Ly49C; Ly49I; LY49I1
UniProt ID: (Mouse) Q64329
Entrez Gene ID: (Mouse) 16640
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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