Invitrogen

Ly-6G Monoclonal Antibody (1A8-Ly6g), eFluor™ 450, eBioscience™

29 Published Figures

37 References

View all (41) Ly-6G antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (29)

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Ly-6G Antibody (48-9668-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse Ly-6C APC (Product # 17-5932-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or Anti-Mouse Ly-6G (Gr-1) eFluor® 450 (right). Total viable cells were used for analysis.

Ly-6G Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

Ly-6G Antibody in Immunocytochemistry (ICC/IF)

Ly-6G Antibody in Immunohistochemistry (IHC)

Ly-6G Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

48-9668-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A8-Ly6g

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637124

Product Specific Information

Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.

Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.

Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Intranasal Delivery of Endothelial Cell-Derived Extracellular Vesicles with Supramolecular Gel Attenuates Myocardial Ischemia-Reperfusion Injury.

International journal of nanomedicine

Wang J,Tan Y,Dai Y,Hu K,Tan X,Jiang S,Li G,Zhang X,Kang L,Wang X,Xu B

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injury.

iScience

Gonzatti MB,Freire BM,Antunes MM,de Menezes GB,Talbot J,Peron JPS,Basso AS,Keller AC

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Neutrophil Extracellular Traps Release following Hypoxic-Ischemic Brain Injury in Newborn Rats Treated with Therapeutic Hypothermia.

International journal of molecular sciences

Bernis ME,Zweyer M,Maes E,Schleehuber Y,Sabir H

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Fri Feb 10 00:00:00 EST 2023

LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer.

Journal for immunotherapy of cancer

Zhao T,Liu S,Hanna NH,Jalal S,Ding X,Wan J,Yan C,Du H

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

GPR84 regulates pulmonary inflammation by modulating neutrophil functions.

Acta pharmacologica Sinica

Wang SW,Zhang Q,Lu D,Fang YC,Yan XC,Chen J,Xia ZK,Yuan QT,Chen LH,Zhang YM,Nan FJ,Xie X

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

48-9668-82 was used in Flow Cytometry to demonstrate that Lin28B promotes lung metastasis of breast cancer by building an immune-suppressive pre-metastatic niche.

Wed Feb 16 00:00:00 EST 2022

Induction of alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19.

Cell host & microbe

Guo Q,Zhao Y,Li J,Liu J,Yang X,Guo X,Kuang M,Xia H,Zhang Z,Cao L,Luo Y,Bao L,Wang X,Wei X,Deng W,Wang N,Chen L,Chen J,Zhu H,Gao R,Qin C,Wang X,You F

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Wed Feb 10 00:00:00 EST 2021

Alterations of macrophage and neutrophil content in skeletal muscle of aged versus young mice.

Muscle & nerve

Kawanishi N,Machida S

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice.

Dose-response : a publication of International Hormesis Society

Rey N,Ebrahimian T,Gloaguen C,Kereselidze D,Magneron V,Bontemps CA,Demarquay C,Olsson G,Haghdoost S,Lehoux S,Ebrahimian TG

48-9668-82 was used in Flow Cytometry to investigate the immunomodulatory response of different doses and dose-rates of irradiation in ApoE-/- mice.

Fri Jun 25 00:00:00 EDT 2021

G-MDSCs promote aging-related cardiac fibrosis by activating myofibroblasts and preventing senescence.

Cell death & disease

Sun SN,Ni SH,Li Y,Liu X,Deng JP,Chen ZX,Li H,Feng WJ,Huang YS,Li DN,Xian SX,Yang ZQ,Wang LJ,Lu L

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

CoV-RBD121-NP Vaccine Candidate Protects against Symptomatic Disease following SARS-CoV-2 Challenge in K18-hACE2 Mice and Induces Protective Responses That Prevent COVID-19-Associated Immunopathology.

Vaccines

DeMarco JK,Royal JM,Severson WE,Gabbard JD,Hume S,Morton J,Swope K,Simpson CA,Shepherd JW,Bratcher B,Palmer KE,Pogue GP

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunocytochemistry

Wed Nov 17 00:00:00 EST 2021

Vaccines

DeMarco JK,Royal JM,Severson WE,Gabbard JD,Hume S,Morton J,Swope K,Simpson CA,Shepherd JW,Bratcher B,Palmer KE,Pogue GP

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunocytochemistry

Wed Nov 17 00:00:00 EST 2021

CCR2 deficiency alters activation of microglia subsets in traumatic brain injury.

Cell reports

Somebang K,Rudolph J,Imhof I,Li L,Niemi EC,Shigenaga J,Tran H,Gill TM,Lo I,Zabel BA,Schmajuk G,Wipke BT,Gyoneva S,Jandreski L,Craft M,Benedetto G,Plowey ED,Charo I,Campbell J,Ye CJ,Panter SS,Nakamura MC,Eckalbar W,Hsieh CL

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Actin fence therapy with exogenous V12Rac1 protects against acute lung injury.

JCI insight

Gusarova GA,Das SR,Islam MN,Westphalen K,Jin G,Shmarakov IO,Li L,Bhattacharya S,Bhattacharya J

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunocytochemistry

Mon Mar 22 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Interleukin-4 Programmed Macrophages Suppress Colitis and Do Not Enhance Infectious-Colitis, Inflammation-Associated Colon Cancer or Airway Hypersensitivity.

Frontiers in immunology

Callejas BE,Blyth GAD,Jendzjowsky N,Wang A,Babbar A,Koro K,Wilson RJA,Kelly MM,Cobo ER,McKay DM

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Fri Dec 17 00:00:00 EST 2021

Interleukin-4 Programmed Macrophages Suppress Colitis and Do Not Enhance Infectious-Colitis, Inflammation-Associated Colon Cancer or Airway Hypersensitivity.

Frontiers in immunology

Callejas BE,Blyth GAD,Jendzjowsky N,Wang A,Babbar A,Koro K,Wilson RJA,Kelly MM,Cobo ER,McKay DM

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Fri Dec 17 00:00:00 EST 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

48-9668 was used in Flow cytometry/Cell sorting to provide demonstration of DA-DRD5 signaling in colonic macrophages controlling the development of colitis by regulating M1/M2 macrophage polarization.

Mon May 17 00:00:00 EDT 2021

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Local sympathetic innervations modulate the lung innate immune responses.

Science advances

Liu T,Yang L,Han X,Ding X,Li J,Yang J

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Fri May 01 00:00:00 EDT 2020

Local sympathetic innervations modulate the lung innate immune responses.

Science advances

Liu T,Yang L,Han X,Ding X,Li J,Yang J

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Fri May 01 00:00:00 EDT 2020

A Simple and Effective Flow Cytometry-Based Method for Identification and Quantification of Tissue Infiltrated Leukocyte Subpopulations in a Mouse Model of Peripheral Arterial Disease.

International journal of molecular sciences

Kumaraswami K,Salei N,Beck S,Rambichler S,Kluever AK,Lasch M,Richter L,Schraml BU,Deindl E

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Tue May 19 00:00:00 EDT 2020

Neutrophil extracellular traps promote scar formation in post-epidural fibrosis.

NPJ Regenerative medicine

Jin Z,Sun J,Song Z,Chen K,Nicolas YSM,Kc R,Ma Q,Liu J,Zhang M

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunohistochemistry

Fri Oct 30 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Bivalent Inhibitor with Selectivity for Trimeric MMP-9 Amplifies Neutrophil Chemotaxis and Enables Functional Studies on MMP-9 Proteoforms.

Cells

Nuti E,Rossello A,Cuffaro D,Camodeca C,Van Bael J,van der Maat D,Martens E,Fiten P,Pereira RVS,Ugarte-Berzal E,Gouwy M,Opdenakker G,Vandooren J

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Tue Jul 07 00:00:00 EDT 2020

IL-1β-MyD88-mTOR Axis Promotes Immune-Protective IL-17A⁺Foxp3⁺ Cells During Mucosal Infection and Is Dysregulated With Aging.

Frontiers in immunology

Bhaskaran N,Faddoul F,Paes da Silva A,Jayaraman S,Schneider E,Mamileti P,Weinberg A,Pandiyan P

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu Q,Xie Z,Qiu M,Shim I,Yang Y,Xie S,Yang Q,Wang D,Chen S,Fan T,Ding B,Guo Z,Adah D,Yao X,Zhang Y,Wu H,Wu Z,Wei C,Wang H,Kim HS,Zou Q,Yan Q,Cai Z,Kim JS,Liu LP,Zhang H,Cao Y

48-9668-82 was used in Flow Cytometry to unravel a promising biphotonic strategy for precision treatment of cancer.

Tue Dec 01 00:00:00 EST 2020

Neutrophil Extracellular Traps Effectively Control Acute Chikungunya Virus Infection.

Frontiers in immunology

Hiroki CH,Toller-Kawahisa JE,Fumagalli MJ,Colon DF,Figueiredo LTM,Fonseca BALD,Franca RFO,Cunha FQ

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunocytochemistry

Mon Nov 16 00:00:00 EST 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

TIV Vaccination Modulates Host Responses to Influenza Virus Infection that Correlate with Protection against Bacterial Superinfection.

Vaccines

Choi A,Christopoulou I,Saelens X,García-Sastre A,Schotsaert M

48-9668 was used in Flow cytometry/Cell sorting to establish a mouse vaccination model that allows control of disease severity after influenza virus infection despite inefficient induction of virus-neutralizing antibody titers by vaccination.

Thu Sep 12 00:00:00 EDT 2019

Transformation of Fonsecaea pedrosoi into sclerotic cells links to the refractoriness of experimental chromoblastomycosis in BALB/c mice via a mechanism involving a chitin-induced impairment of IFN-γ production.

PLoS neglected tropical diseases

Dong B,Tong Z,Li R,Chen SC,Liu W,Liu W,Chen Y,Zhang X,Duan Y,Li D,Chen L

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation.

Cell host & microbe

Gimblet C,Meisel JS,Loesche MA,Cole SD,Horwinski J,Novais FO,Misic AM,Bradley CW,Beiting DP,Rankin SC,Carvalho LP,Carvalho EM,Scott P,Grice EA

48-9668-82 was used in Flow Cytometry to investigate the role of skin microbiota in cutaneous leishmaniasis and found that human patients infected with Leishmania braziliensis develop dysbiotic skin microbiota, characterized by increases in the abundance of Staphylococcus and/or Streptococcus.

Wed Jul 12 00:00:00 EDT 2017

Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration.

Cell research

Cai Z,Zhang A,Choksi S,Li W,Li T,Zhang XM,Liu ZG

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

The spleen contributes importantly to myocardial infarct exacerbation during post-ischemic reperfusion in mice via signaling between cardiac HMGB1 and splenic RAGE.

Basic research in cardiology

Tian Y,Pan D,Chordia MD,French BA,Kron IL,Yang Z

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Immunofluorescence

Tue Nov 01 00:00:00 EDT 2016

ATP-Induced Inflammasome Activation and Pyroptosis Is Regulated by AMP-Activated Protein Kinase in Macrophages.

Frontiers in immunology

Zha QB,Wei HX,Li CG,Liang YD,Xu LH,Bai WJ,Pan H,He XH,Ouyang DY

Published figure using Ly-6G monoclonal antibody (Product # 48-9668-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Bioinformatics

Protein Aliases: Ly-6G; Ly-6G.1; Lymphocyte antigen 6G

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Gene Aliases: Gr-1; Gr1; Ly-6G; Ly6g

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UniProt ID: (Mouse) P35461

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Entrez Gene ID: (Mouse) 546644

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Performance Guarantee

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Ly-6G Monoclonal Antibody (1A8-Ly6g), eFluor™ 450, eBioscience™

Ly-6G Antibody (48-9668-82) in Flow

Product Details

48-9668-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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