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Lysine Acetylated Antibody (600-401-939) in WB
Affinity Purified anti-Acetylated Lysine (AcK) antibody (Product # 600-401-939) is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).
Product Details
600-401-939
Product Specifications
Species Reactivity
Many
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
Store at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).
Target Information
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl Lysine; Acetylated Lysine; Acetyllysine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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