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M-CSF Antibody (500-P62GBT-25UG) in ELISA
Sandwich ELISA: To detect mM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of M-CSF Polyclonal Antibody, Biotin (Product # 500-P62GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech M-CSF Polyclonal Antibody (Product # 500-P62G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mM-CSF.
Product Details
500-P62GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine M-CSF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930663
Product Specific Information
AA Sequence of recombinant protein: MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine M-CSF. Anti-Murine M-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine M-CSF (500-P62G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mM-CSF.
Western Blot: To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P62GBT-1MG will be provided as 2 x 500 µg
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSF-1; H-MCSF; M-MCSF; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
Gene Aliases: C87615; Csf1; Csfm; MCSF; op
UniProt ID: (Mouse) P07141
Entrez Gene ID: (Mouse) 12977
ossification
positive regulation of cell-matrix adhesion
osteoclast proliferation
immune system process
developmental process involved in reproduction
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of macrophage chemotaxis
macrophage differentiation
regulation of ossification
osteoclast differentiation
positive regulation of cell migration
positive regulation of cellular protein metabolic process
positive regulation of mononuclear cell proliferation
macrophage colony-stimulating factor signaling pathway
positive regulation of multicellular organism growth
odontogenesis
positive regulation of odontogenesis of dentin-containing tooth
innate immune response
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of osteoclast differentiation
positive regulation of protein kinase activity
positive regulation of Ras protein signal transduction
homeostasis of number of cells within a tissue
branching involved in mammary gland duct morphogenesis
mammary gland fat development
mammary duct terminal end bud growth
positive regulation of macrophage colony-stimulating factor signaling pathway
positive regulation of microglial cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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