Invitrogen

M-CSF Polyclonal Antibody

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Antibody Testing Data (3)
Published Figures (4)

M-CSF Antibody in Immunohistochemistry (IHC)

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M-CSF Antibody (PA5-42558) in IHC

Immunohistochemistry analysis of human placenta tissue using an anti-CSF1 polyclonal antibody (Product # PA5-42558).

M-CSF Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

PA5-42558

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human CSF1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2609577

Product Specific Information

Peptide sequence: SGSVLPLGEL EGRRSTRDRR SPAEPEGGPA SEGAARPLPR FNSVPLTDTG

Sequence homology: Dog: 85%; Guinea Pig: 79%; Human: 100%; Mouse: 86%; Pig: 85%; Rat: 86%

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spinal Cord Stimulation Alleviates Neuropathic Pain by Attenuating Microglial Activation via Reducing Colony-Stimulating Factor 1 Levels in the Spinal Cord in a Rat Model of Chronic Constriction Injury.

Anesthesia and analgesia

Sun C,Tao X,Wan C,Zhang X,Zhao M,Xu M,Wang P,Liu Y,Wang C,Xi Q,Song T

PA5-42558 was used in Immunohistochemistry-immunofluorescence to demonstrate that Spinal cord stimulation (SCS) reduces microglial activation in the spinal cord and alleviates chronic neuropathic pain (NP), at least in part by inhibiting the release of colony-stimulating factor 1 (CSF1) from the dorsal root ganglion ipsilateral to nerve injury.

Fri Jul 01 00:00:00 EDT 2022

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

PA5-42558 was used in Western Blot, Immunohistochemistry (Paraffin) to suggest senescent tumor cells generate a cytokine barrier protecting nonsenescent tumor cells from immune attack and provide a new target for overcoming the immunotherapy resistance of CRC.

Mon Feb 01 00:00:00 EST 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 EST 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 EST 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Mon Feb 01 00:00:00 EST 2021

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

PA5-42558 was used in Immunohistochemistry-immunofluorescence to analyse schwann cells phenotypes in sciatic nerves of amyotrophic lateral sclerosis patients and paralytic SOD1G93A rats to identify if similar and specific reactive schwann cells phenotypes exist.

Mon Jun 01 00:00:00 EDT 2020

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

Mon Jun 01 00:00:00 EDT 2020

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

Mon Jun 01 00:00:00 EDT 2020

Regulators of TNFα mediated insulin resistance elucidated by quantitative proteomics.

Scientific reports

Mohallem R,Aryal UK

Published figure using M-CSF polyclonal antibody (Product # PA5-42558) in Western Blot

Mon Nov 30 00:00:00 EST 2020

Regulators of TNFα mediated insulin resistance elucidated by quantitative proteomics.

Scientific reports

Mohallem R,Aryal UK

Published figure using M-CSF polyclonal antibody (Product # PA5-42558) in Western Blot

Mon Nov 30 00:00:00 EST 2020

Bioinformatics

Protein Aliases: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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Gene Aliases: CSF-1; CSF1; MCSF

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UniProt ID: (Human) P09603

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Entrez Gene ID: (Human) 1435

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Function(s)

cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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M-CSF Polyclonal Antibody

M-CSF Antibody (PA5-42558) in IHC

Product Details

PA5-42558

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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