Invitrogen

M-CSF Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-95732

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-190 of human CSF1 (NP_7573511)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.78 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807534

Product Specific Information

Immunogen sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCN; Positive Samples: BxPC-3, Jurkat, K-562; Cellular Location: Cell membrane, Single-pass type I membrane protein, extracellular space

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MCSF; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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Gene Aliases: C87615; CSF-1; CSF1; Csfm; MCSF; op

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UniProt ID: (Human) P09603, (Mouse) P07141

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Entrez Gene ID: (Human) 1435, (Mouse) 12977, (Rat) 78965

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Function(s)
cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity
Process(es)
ossification positive regulation of cell-matrix adhesion osteoclast proliferation immune system process developmental process involved in reproduction inflammatory response transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation positive regulation of cell proliferation positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of macrophage chemotaxis macrophage differentiation regulation of ossification osteoclast differentiation positive regulation of cell migration positive regulation of cellular protein metabolic process positive regulation of mononuclear cell proliferation macrophage colony-stimulating factor signaling pathway positive regulation of multicellular organism growth odontogenesis positive regulation of odontogenesis of dentin-containing tooth innate immune response positive regulation of macrophage differentiation positive regulation of monocyte differentiation positive regulation of osteoclast differentiation positive regulation of protein kinase activity positive regulation of Ras protein signal transduction homeostasis of number of cells within a tissue branching involved in mammary gland duct morphogenesis mammary gland fat development mammary duct terminal end bud growth positive regulation of macrophage colony-stimulating factor signaling pathway positive regulation of microglial cell migration regulation of macrophage derived foam cell differentiation hemopoiesis cell differentiation monocyte activation
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